Udvardy M, Schwartzott D, Jackson K, McKee P A
Second Department of Medicine, University Medical School, Debrecen, Hungary.
Blood Coagul Fibrinolysis. 1995 Feb;6(1):11-6. doi: 10.1097/00001721-199502000-00002.
The aim of this study was to synthesize and investigate hybrid peptides which contain the RGD (Arg-Gly-Asp) sequence coupled with lysine residues in special arrangements (antiplasmin carboxyterminal peptide) in an effort to simultaneously inhibit platelet aggregation and promote fibrinolysis. The in vitro haemostatic modifying properties of the synthesized peptides were tested by ADP-induced platelet aggregation, plasmin-generation tests and fibrin-clot lysis assays. The hybrid peptide RGDFAP, composed of RGDF (Arg-Gly-Asp-Phe) coupled to a synthetic peptide residue of the carboxyterminal part of antiplasmin (AP26) inhibited platelet activation and increased plasmin generation and in vitro fibrin-clot lysis.
本研究的目的是合成并研究含有RGD（精氨酸-甘氨酸-天冬氨酸）序列且赖氨酸残基以特殊排列方式结合的杂合肽（抗纤溶酶羧基末端肽），以期同时抑制血小板聚集并促进纤维蛋白溶解。通过ADP诱导的血小板聚集试验、纤溶酶生成试验和纤维蛋白凝块溶解试验，对合成肽的体外止血修饰特性进行了测试。由RGDF（精氨酸-甘氨酸-天冬氨酸-苯丙氨酸）与抗纤溶酶羧基末端部分的合成肽残基（AP26）组成的杂合肽RGDFAP，抑制了血小板活化，增加了纤溶酶生成以及体外纤维蛋白凝块溶解。
