Markham R, Young L, Fraser I S
Department of Obstetrics and Gynaecology, Queen Elizabeth II Research Institute for Mothers and Infants, University of Sydney, NSW, Australia.
Eur Cytokine Netw. 1995 Jan-Feb;6(1):49-54.
An immunoassay for tumour necrosis factor alpha (TNF-alpha) is described using a sandwich system which employs a mouse monoclonal as the capture antibody and a polyclonal rabbit anti-TNF-alpha as the detection antibody. The assay utilises a novel enzyme cycling system to amplify the colourimetric signal generated by alkaline phosphatase in the enzyme immunoassay. The assay sensitivity is increased by the use of the AMPAK system. The detection limit of the assay is in the order of 1 pg/ml which is many times greater than is possible with conventional methods of measurement. The advantages of the enzyme-amplified method will be particularly useful in the quantitative determination of low levels of TNF-alpha in body fluids. This assay system was used to measure peripheral serum TNF levels in a group of women with non-malignant gynaecological conditions, the mean TNF-alpha concentration in these subjects was 10 pg/ml.
本文描述了一种用于检测肿瘤坏死因子α（TNF-α）的免疫测定法，该方法采用夹心系统，以小鼠单克隆抗体作为捕获抗体，以兔抗TNF-α多克隆抗体作为检测抗体。该测定法利用一种新型酶循环系统来放大酶免疫测定中碱性磷酸酶产生的比色信号。通过使用AMPAK系统提高了测定的灵敏度。该测定法的检测限约为1 pg/ml，比传统测量方法的检测限高许多倍。酶放大法的优点在定量测定体液中低水平的TNF-α时将特别有用。该测定系统用于测量一组患有非恶性妇科疾病的女性外周血清TNF水平，这些受试者的平均TNF-α浓度为10 pg/ml。
