Yazaki I
Department of Biology, Faculty of Science, Tokyo Metropolitan University, Japan.
Zoolog Sci. 1995 Feb;12(1):105-12. doi: 10.2108/zsj.12.105.
Metamorphosis of the sea urchin, Hemicentrotus pulcherrimus, can be induced by L-glutamine as reported previously for Pseudocentrotus depressus [15]. To analyze more precisely the process of metamorphosis induced by L-glutamine, the development of the echinus rudiment (ER) was classified into six stages. The stage at which the larvae underwent the normal metamorphosis by glutamine treatment was confirmed. The time of the glutamine treatment required for metamorphosis (the eversion of ER) was over 10 hr but treatment for more than 25 hr tended to decrease the number of metamorphosed larvae, although the larval arms had mostly been resorbed. The time of glutamine treatment to induce the metamorphosis, depended on the development of ER; more time was required for the younger larvae and less time for the older. The high mitotic activities observed in the cells of the ciliary bands were markedly decreased in the glutamine-treated larvae to metamorphose. These findings suggested that a degenerative process of metamorphosis including cell death is induced by L-glutamine.
如之前关于虾夷马粪海胆（Pseudocentrotus depressus）的报道所述，谷氨酰胺可诱导光棘球海胆（Hemicentrotus pulcherrimus）变态。为更精确地分析谷氨酰胺诱导的变态过程，将海胆原基（ER）的发育分为六个阶段。确定了幼虫通过谷氨酰胺处理进行正常变态的阶段。变态所需的谷氨酰胺处理时间（ER外翻）超过10小时，但处理超过25小时往往会减少变态幼虫的数量，尽管幼虫的臂大多已被吸收。诱导变态的谷氨酰胺处理时间取决于ER的发育；较年幼的幼虫需要更多时间，而较年长的幼虫需要较少时间。在谷氨酰胺处理以进行变态的幼虫中，观察到的纤毛带细胞中的高有丝分裂活性明显降低。这些发现表明，谷氨酰胺可诱导包括细胞死亡在内的变态退化过程。
