Rabbitts P, Impey H, Heppell-Parton A, Langford C, Tease C, Lowe N, Bailey D, Ferguson-Smith M, Carter N
MRC Radiotherapeutics Unit, MRC Centre, Cambridge, UK.
Nat Genet. 1995 Apr;9(4):369-75. doi: 10.1038/ng0495-369.
Chromosomes from antigen stimulated B-cells from spleens of inbred mice have been separated using flow cytometry into 18 distinguishable peaks. Using locus-specific oligonucleotides and fluorescence in situ hybridization to banded metaphase spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 12, 16, 17, 18, 19, X and Y. The remaining six chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 with 15, were resolved by flow sorting chromosomes from mice carrying an appropriate homozygous translocation and 4, 5 and 14 have been isolated in this way. This is the first demonstration of how a complete set of mouse chromosome paints can be produced.
利用流式细胞术，已将近交系小鼠脾脏中抗原刺激的B细胞的染色体分离成18个可区分的峰。使用位点特异性寡核苷酸和荧光原位杂交技术对带型中期染色体进行分析，鉴定出了15条单条染色体：1、2、3、6、7、8、9、11、12、16、17、18、19、X和Y。其余6条染色体以成对形式出现在三个峰中，即4与5、10与13、14与15，通过对携带适当纯合易位的小鼠的染色体进行流式分选得以区分，4、5和14号染色体已通过这种方式分离出来。这是首次展示如何制备一套完整的小鼠染色体涂染探针。
