Kasai H, Kawamoto K
Department of Neurosurgery, Kansai Medical University.
Noshuyo Byori. 1995 Mar;12(1):75-82.
In the present study, an analysis was made of chromosomal aberrations in brain tumors using the fluorescence in situ hybridization (FISH) technique. At the same time DNA histograms were obtained by flow cytometry (FCM) to make a comparative study of histological malignancy and prognosis. The subjects included 30 gliomas (7 of astrocytoma grade II, 15 of anaplastic astrocytoma (grade III), 8 of glioblastoma (grade IV)) and 26 meningiomas. In the study of FISH, DNA probes for chromosomes No. 7, 9, 10, and 17, were used to cause a reaction with chromosome No. 22 added for meningiomas. In the study with FCM, DNA index from DNA histogram was calculated using lymphocytes as the internal standard. In gliomas as a whole, chromosomes No. 7 and 17 showed high values, whereas, chromosomes No. 9 and 10 low values. Analysis by the grades of glioma showed that compared with gliomas of other grades, grade IV gliomas were higher for chromosome No. 17 and lower for chromosome No. 10. In meningiomas, while many cases showed a low value for chromosome No. 22, most cases of recurrent and atypical meningiomas showed a high value for chromosome No. 17. In gliomas, DNA index showed a correlation with the grade, and a positive correlation particularly with chromosome No. 17 in FISH. Recurrent and atypical meningiomas had a high DNA index.
在本研究中，运用荧光原位杂交（FISH）技术对脑肿瘤中的染色体畸变进行了分析。同时，通过流式细胞术（FCM）获得DNA直方图，以对组织学恶性程度和预后进行比较研究。研究对象包括30例胶质瘤（7例二级星形细胞瘤、15例间变性星形细胞瘤（三级）、8例胶质母细胞瘤（四级））和26例脑膜瘤。在FISH研究中，使用7号、9号、10号和17号染色体的DNA探针，并针对脑膜瘤添加22号染色体以引发反应。在FCM研究中，以淋巴细胞作为内标，根据DNA直方图计算DNA指数。在整体胶质瘤中，7号和17号染色体显示高值，而9号和10号染色体显示低值。按胶质瘤分级分析表明，与其他分级的胶质瘤相比，四级胶质瘤的17号染色体值较高，10号染色体值较低。在脑膜瘤中，虽然许多病例的22号染色体值较低，但大多数复发性和非典型脑膜瘤的17号染色体值较高。在胶质瘤中，DNA指数与分级相关，在FISH中尤其与17号染色体呈正相关。复发性和非典型脑膜瘤具有较高的DNA指数。
