Pham-Huy C, Radenen B, Sahui-Gnassi A, Claude J R
Laboratoire de Toxicologie (EA 207), Faculté de Pharmacie, Université René Descartes, Paris V, France.
J Chromatogr B Biomed Appl. 1995 Mar 10;665(1):125-32. doi: 10.1016/0378-4347(94)00511-3.
The determination of propranolol enantiomers in microsamples of human plasma and urine by HPLC using a chiral stationary phase is described. After extraction from 200 microliters of plasma or urine with racemic alprenolol as internal standard (I.S.), the enantiomers are separated on a beta-cyclodextrin column with a polar organic mobile phase and determined by fluorescence detection. The retention times of I.S. and propranolol enantiomers are about 12-13 min and 16-18 min, respectively. Peak resolutions are 1.4 for I.S. and 2.2 for propranolol. The use of alprenolol as I.S. improves significantly the coefficients of variation (C.V.: 0.6-4.2%). Sensitivity is approximately 1.5 ng/ml per propranolol enantiomer. The assay is applied to pharmacokinetic studies of racemic propranolol in human biological fluids. The (S)-propranolol levels are always higher than the (R)-antipode concentrations in plasma and urine.
描述了使用手性固定相通过高效液相色谱法测定人血浆和尿液微量样品中普萘洛尔对映体的方法。以外消旋阿普洛尔为内标（I.S.）从200微升血浆或尿液中萃取后，对映体在β-环糊精柱上用极性有机流动相进行分离，并通过荧光检测进行测定。内标和普萘洛尔对映体的保留时间分别约为12 - 13分钟和16 - 18分钟。内标的峰分辨率为1.4，普萘洛尔的峰分辨率为2.2。使用阿普洛尔作为内标显著提高了变异系数（C.V.：0.6 - 4.2%）。灵敏度约为每普萘洛尔对映体1.5纳克/毫升。该测定法应用于消旋普萘洛尔在人体生物流体中的药代动力学研究。在血浆和尿液中，（S）-普萘洛尔的水平总是高于（R）-对映体的浓度。
