气相色谱法同时测定人血浆中咪达唑仑和氟马西尼的浓度

Simultaneous determination of midazolam and flumazenil concentrations in human plasma by gas chromatography.

作者信息

Fisher L E, Perch S, Bonfiglio M F, Geers S M

机构信息

Department of Pharmacy, Summa Health System, Akron, OH 44309, USA.

出版信息

J Chromatogr B Biomed Appl. 1995 Mar 10;665(1):217-21. doi: 10.1016/0378-4347(94)00510-c.

DOI:10.1016/0378-4347(94)00510-c

PMID:7795795

Abstract

A gas chromatographic method for the simultaneous determination of midazolam and flumazenil concentrations in human plasma has been developed, using clinazolam as the internal standard. A mixture of chloroform and ethyl acetate (80:20) was used to extract the two compounds. A 2-microliters volume of the reconstituted sample was injected using a 1:20 split injection mode. Intra-day coefficients of variation ranged from 2 to 6.9%. The assay was linear over the range 3-1000 ng/ml. This assay was subsequently used to analyze samples from a human pharmacokinetic study.

摘要

已开发出一种气相色谱法，以氯硝西泮为内标，同时测定人血浆中咪达唑仑和氟马西尼的浓度。使用氯仿和乙酸乙酯（80:20）的混合物提取这两种化合物。采用1:20分流进样模式注入2微升复溶后的样品。日内变异系数范围为2%至6.9%。该测定法在3 - 1000 ng/ml范围内呈线性。此测定法随后用于分析一项人体药代动力学研究的样本。