So D Y, Temkin R J, Lea P J
Scanning Electron Microscopy Core Facility, Faculty of Medicine, University of Toronto, Ontario, Canada.
Scanning. 1995 May-Jun;17(3):161-70. doi: 10.1002/sca.4950170307.
Brefeldin A (BFA) has been reported to cause disassembly of the Golgi. We have used three-dimensional (3-D) high-resolution scanning electron microscopy (HRSEM) to investigate these effects in human skin fibroblast cells. The spontaneous reassembly during prolonged exposure to BFA and some effects of forskolin were observed. A BFA concentration of 5 micrograms/ml caused Golgi complexes to become vesicular, resulting in a progressive decrease in the size of the Golgi. Morphologic changes were visible within 2 min of BFA incubation, and by 30 min no identifiable Golgi could be found. Spontaneous reassembly of the Golgi apparatus upon the removal of the BFA or with continued long-term exposure with BFA could not be confirmed. Preliminary experiments with forskolin were not effective in reversing or inhibiting the effects of BFA in human fibroblast cells grown in culture. This inability for spontaneous reassembly and nonreversal by forskolin may reflect a differential effect of BFA in various cell types. HRSEM has proven to be useful for observing 3-D morphologic effects of BFA in Golgi.
据报道，布雷菲德菌素A（BFA）可导致高尔基体解体。我们使用三维（3-D）高分辨率扫描电子显微镜（HRSEM）来研究其对人皮肤成纤维细胞的这些影响。观察到在长时间暴露于BFA期间的自发重新组装以及福斯高林的一些作用。5微克/毫升的BFA浓度会使高尔基体复合物变成小泡状，导致高尔基体的大小逐渐减小。在BFA孵育2分钟内即可观察到形态变化，到30分钟时已找不到可识别的高尔基体。去除BFA后或持续长期暴露于BFA时高尔基体的自发重新组装无法得到证实。用福斯高林进行的初步实验在逆转或抑制培养的人成纤维细胞中BFA的作用方面无效。这种无法自发重新组装以及福斯高林无法逆转的情况可能反映了BFA在各种细胞类型中的不同作用。HRSEM已被证明可用于观察BFA对高尔基体的三维形态学影响。
