对白化大鼠中向腹外侧膝状体核（vLGN）和外侧后核（LPN）而非背外侧膝状体核（dLGN）和上丘（SC）同侧投射的视网膜神经节细胞的逆行双标记研究。

A retrograde double-labelling study of retinal ganglion cells that project ipsilaterally to vLGN and LPN rather than dLGN and SC, in albino rat.

作者信息

Ahmed A K, Dong K, Yamadori T

机构信息

First Department of Anatomy, Kobe University School of Medicine, Japan.

出版信息

Brain Res. 1995 Mar 20;674(2):275-82. doi: 10.1016/0006-8993(94)01452-n.

DOI:10.1016/0006-8993(94)01452-n

PMID:7796107

Abstract

We studied ipsilaterally projecting, double-labeled retinal ganglion cells that have bifurcating axons by retrograde fluorescent double-labeling in albino rats. Ten albino (Wistar, Japan Ceca) rats of either sex, weighing 350-400 g were used. With the rats in a state of deep anesthesia, we pressure-injected 0.02 microliter of 15% Evans blue (EB) into the right ventral lateral geniculate nucleus (vLGN), and 4% Fluoro-gold (FG) iontophoretically into the right posterior lateral thalamic nucleus (LP). The animals were perfused with formol-saline 48-72 h later and both the brain and eyes were exercised. The brain was sectioned coronally, and each retina was removed and mounted flat on a glass slide. Double-labeled cells were found in the ventral temporal crescent of the retina. In one animal and total number of ipsilaterally labeled cells was 566, and the percentage of double-labeled vLGN and LP projecting cells, single-labeled vLGN projecting cells, and single-labeled LP projecting cells were 29.8, 58.8 and 11.3, respectively.

摘要

我们通过对白化大鼠进行逆行荧光双标记，研究了具有分叉轴突的同侧投射双标记视网膜神经节细胞。使用了10只体重350 - 400克的白化（Wistar，日本Ceca）大鼠，雌雄不限。在大鼠处于深度麻醉状态下，我们将0.02微升15%伊文思蓝（EB）经压力注射到右侧腹外侧膝状核（vLGN），并将4%荧光金（FG）通过离子电泳法注入右侧丘脑后外侧核（LP）。48 - 72小时后用甲醛生理盐水灌注动物，并取出大脑和眼睛。将大脑冠状切片，每个视网膜取出并平铺在载玻片上。在视网膜的腹侧颞侧新月区发现了双标记细胞。在一只动物中，同侧标记细胞总数为566个，双标记vLGN和LP投射细胞、单标记vLGN投射细胞以及单标记LP投射细胞的百分比分别为29.8%、58.8%和11.3%。