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Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli.

作者信息

Merritt E A, Sarfaty S, Pizza M, Domenighini M, Rappuoli R, Hol W G

出版信息

Nat Struct Biol. 1995 Apr;2(4):269-72. doi: 10.1038/nsb0495-269.

DOI:10.1038/nsb0495-269
PMID:7796260
Abstract
摘要

相似文献

1
Mutation of a buried residue causes loss of activity but no conformational change in the heat-labile enterotoxin of Escherichia coli.大肠杆菌不耐热肠毒素中一个埋藏残基的突变导致活性丧失,但构象无变化。
Nat Struct Biol. 1995 Apr;2(4):269-72. doi: 10.1038/nsb0495-269.
2
Structure of partially-activated E. coli heat-labile enterotoxin (LT) at 2.6 A resolution.分辨率为2.6埃的部分激活的大肠杆菌不耐热肠毒素(LT)的结构。
FEBS Lett. 1994 Jan 3;337(1):88-92. doi: 10.1016/0014-5793(94)80635-7.
3
Site-directed mutagenic alteration of potential active-site residues of the A subunit of Escherichia coli heat-labile enterotoxin. Evidence for a catalytic role for glutamic acid 112.
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Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli.来自大肠杆菌的一种霍乱毒素相关热不稳定肠毒素的晶体结构。
Nature. 1991 May 30;351(6325):371-7. doi: 10.1038/351371a0.
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Crystal structure of a non-toxic mutant of heat-labile enterotoxin, which is a potent mucosal adjuvant.不耐热肠毒素无毒突变体的晶体结构,该突变体是一种有效的黏膜佐剂。
Protein Sci. 1997 Dec;6(12):2650-4. doi: 10.1002/pro.5560061220.
6
Crystal structure of a new heat-labile enterotoxin, LT-IIb.新型不耐热肠毒素LT-IIb的晶体结构
Structure. 1996 Jun 15;4(6):665-78. doi: 10.1016/s0969-2126(96)00073-1.
7
A pH-dependent conformational change in the B-subunit pentamer of Escherichia coli heat-labile enterotoxin: structural basis and possible functional role for a conserved feature of the AB5 toxin family.大肠杆菌不耐热肠毒素B亚基五聚体中pH依赖性构象变化:AB5毒素家族保守特征的结构基础及可能的功能作用
Biochemistry. 1996 Dec 17;35(50):16069-76. doi: 10.1021/bi961865l.
8
Role of trypsin-like cleavage at arginine 192 in the enzymatic and cytotonic activities of Escherichia coli heat-labile enterotoxin.精氨酸192处类似胰蛋白酶的切割在大肠杆菌热不稳定肠毒素的酶活性和细胞毒性活性中的作用
Infect Immun. 1994 Oct;62(10):4270-8. doi: 10.1128/iai.62.10.4270-4278.1994.
9
The Arg7Lys mutant of heat-labile enterotoxin exhibits great flexibility of active site loop 47-56 of the A subunit.不耐热肠毒素的Arg7Lys突变体在A亚基的活性位点环47-56处表现出极大的灵活性。
Biochemistry. 1995 Sep 5;34(35):10996-1004. doi: 10.1021/bi00035a005.
10
Heat-labile enterotoxin crystal forms with variable A/B5 orientation. Analysis of conformational flexibility.
FEBS Lett. 1992 Jun 29;305(2):81-5. doi: 10.1016/0014-5793(92)80869-i.

引用本文的文献

1
Identification of motifs in cholera toxin A1 polypeptide that are required for its interaction with human ADP-ribosylation factor 6 in a bacterial two-hybrid system.在细菌双杂交系统中鉴定霍乱毒素A1多肽中与人类ADP-核糖基化因子6相互作用所需的基序。
Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14662-7. doi: 10.1073/pnas.011442598.
2
Effects of site-directed mutagenesis of Escherichia coli heat-labile enterotoxin on ADP-ribosyltransferase activity and interaction with ADP-ribosylation factors.大肠杆菌不耐热肠毒素的定点诱变对ADP-核糖基转移酶活性及与ADP-核糖基化因子相互作用的影响
Infect Immun. 1999 Jan;67(1):259-65. doi: 10.1128/IAI.67.1.259-265.1999.
3
Mutations in the A subunit affect yield, stability, and protease sensitivity of nontoxic derivatives of heat-labile enterotoxin.
A亚基中的突变会影响不耐热肠毒素无毒衍生物的产量、稳定性和蛋白酶敏感性。
Infect Immun. 1996 Dec;64(12):5434-8. doi: 10.1128/iai.64.12.5434-5438.1996.