Nomiyama T, Omae K, Uemura T, Nakashima H, Takebayashi T, Ishizuka C, Yamazaki K, Sakurai H
Department of Preventive Medicine and Public Health, Keio University, Tokyo, Japan.
Sangyo Eiseigaku Zasshi. 1995 May;37(3):157-60. doi: 10.1539/sangyoeisei.37.3_157.
In order to clarify the acute and subacute toxicity of diborane (B2H6, CAS: 19287-45-7) at low concentrations, male ICR mice were exposed to diborane for 1, 2, 4 or 8 h at concentrations of 1 or 5 ppm (phase I study), and for 6 h/day, 5 days/wk, over 2 or 4 wk at concentrations of 0.02 or 0.7 ppm (phase II study). Hematological and biochemical tests, and histopathological examinations of the cornea, nasal mucosa, respiratory tract and lung were carried out. All mice in both studies survived until they were sacrificed. In the phase I study, lung weight increased significantly in mice exposed to 5 ppm of diborane for 8 h. Histopathologically diffuse panbronchiolitis-like lesion was observed in mice exposed to 5 ppm of diborane for 2, 4 or 8 h. In the phase II study, slight infiltration of polymorphous neutrophil was observed mainly in the peribronchiolar region in mice exposed to 0.2 ppm or 0.7 ppm of diborane for 2 or 4 wk. In both studies, hematological and biochemical examinations failed to reveal any exposure-related changes. These results suggest that no-observed-effect level of diborane inhalation on the respiratory organs were 1 ppm in acute exposure, but 0.2 ppm of diborane inhalation for 2 or 4 wk seems to be unsafe.
为阐明低浓度乙硼烷（B2H6，化学物质登记号：19287-45-7）的急性和亚急性毒性，将雄性ICR小鼠暴露于浓度为1或5 ppm的乙硼烷中1、2、4或8小时（I期研究），并以浓度0.02或0.7 ppm每周5天、每天6小时暴露2或4周（II期研究）。进行了血液学和生化检测，以及角膜、鼻黏膜、呼吸道和肺的组织病理学检查。两项研究中的所有小鼠在处死前均存活。在I期研究中，暴露于5 ppm乙硼烷8小时的小鼠肺重量显著增加。组织病理学观察发现，暴露于5 ppm乙硼烷2、4或8小时的小鼠出现弥漫性细支气管炎样病变。在II期研究中，暴露于0.2 ppm或0.7 ppm乙硼烷2或4周的小鼠主要在细支气管周围区域观察到多形核中性粒细胞轻度浸润。在两项研究中，血液学和生化检查均未发现任何与暴露相关的变化。这些结果表明，急性暴露时乙硼烷吸入对呼吸器官的未观察到有害作用水平为1 ppm，但吸入0.2 ppm乙硼烷2或4周似乎不安全。
