Nakamura K, Antoku S
Department of Experimental Radiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer Biochem Biophys. 1994 Apr;14(1):15-21.
Protein kinases play an important role in the response of mammalian cells to ionizing radiation. In this study, we examined the effect of staurosporine, a potent inhibitor of protein kinases, on cell survival after X-irradiation, using the normal rat fibroblast line 3Y1. Treatment with 30 ng/ml staurosporine 1 h before irradiation resulted in the increase in survival of 3Y1 cells. This phenomenon was drug dose-dependent and maximal reduction of radiation-induced cell killing occurred when more than, or equal to, 30 ng/ml staurosporine was added. In contrast, treatment with 30 ng/ml staurosporine after irradiation did not increase cell survival. There was no change in cell cycle distribution by treatment for 1 h with 30 ng/ml staurosporine. These data suggest that the inhibition of protein kinase activities by staurosporine influences the radiosensitivity of 3Y1 cells.
蛋白激酶在哺乳动物细胞对电离辐射的反应中起重要作用。在本研究中，我们使用正常大鼠成纤维细胞系3Y1，研究了蛋白激酶的强效抑制剂星形孢菌素对X射线照射后细胞存活的影响。照射前1小时用30 ng/ml星形孢菌素处理导致3Y1细胞存活率增加。这种现象呈药物剂量依赖性，当添加大于或等于30 ng/ml星形孢菌素时，辐射诱导的细胞杀伤作用最大程度降低。相比之下，照射后用3 ng/ml星形孢菌素处理并未增加细胞存活率。用30 ng/ml星形孢菌素处理1小时对细胞周期分布没有影响。这些数据表明，星形孢菌素对蛋白激酶活性的抑制影响了3Y1细胞的放射敏感性。
