人类肝脏组织中的端粒酶活性：慢性肝病与肝细胞癌的比较

Telomerase activity in human liver tissues: comparison between chronic liver disease and hepatocellular carcinomas.

作者信息

Tahara H, Nakanishi T, Kitamoto M, Nakashio R, Shay J W, Tahara E, Kajiyama G, Ide T

机构信息

Department of Cellular and Molecular Biology, Hiroshima University School of Medicine, Japan.

出版信息

Cancer Res. 1995 Jul 1;55(13):2734-6.

PMID:7796395

Abstract

Telomerase activity was examined in 105 frozen samples from human normal liver tissues, chronic liver disease, and hepatocellular carcinoma (HCC). Telomerase activity was positive in 28 of 33 HCC tissues regardless of tumor stage or size. Telomerase was expressed in 15 of 18 differentiated HCC nodules smaller than 3 cm. HCC tissues from all eight hepatitis B virus-positive patients were telomerase positive, while telomerase activity was not detected in normal liver tissues (0 of 4). Weak telomerase activity was only detected in 1 of 22 nontumor liver tissues from HCC patients. Interestingly, in 19 of 38 hepatitis tissues and 6 of 8 cirrhotic liver tissues from apparently cancer-free patients, very weak telomerase activity was detected. These results indicate that the expression of telomerase may play a crucial role in hepatocarcinogenesis.

摘要

对来自人类正常肝组织、慢性肝病和肝细胞癌（HCC）的105份冷冻样本进行了端粒酶活性检测。33份HCC组织中有28份端粒酶活性呈阳性，无论肿瘤分期或大小如何。18个小于3 cm的分化型HCC结节中有15个表达端粒酶。所有8例乙型肝炎病毒阳性患者的HCC组织端粒酶均为阳性，而正常肝组织中未检测到端粒酶活性（4例均为阴性）。仅在22例HCC患者的非肿瘤肝组织中有1例检测到微弱的端粒酶活性。有趣的是，在38例肝炎组织中的19例以及8例明显无癌患者的肝硬化肝组织中的6例中，检测到非常微弱的端粒酶活性。这些结果表明，端粒酶的表达可能在肝癌发生过程中起关键作用。