Okuyama R, Yanai N, Obinata M
Department of Cell Biology, Tohoku University, Sendai, Japan.
Exp Cell Res. 1995 Jun;218(2):424-9. doi: 10.1006/excr.1995.1175.
Stromal cell lines were established from bone marrow of temperature-sensitive T-antigen gene transgenic mice. These stromal cell lines consisted of fibroblasts, endothelial cells, and preadipocytes. We found that these stromal cell lines exhibited phenotypic changes depending on the inactivation of T-antigen and growth condition; one preadipocyte line was induced toward adipocytes and osteogenic cells, and several preadipocyte and endothelial cell lines were induced toward muscle cells and adipocytes. Some cell lines showed bipotential characters. These results indicated that stromal cells consisting of bone marrow hematopoietic microenvironment are derived from multipotent mesenchymal stem cells.
基质细胞系是从温度敏感型T抗原基因转基因小鼠的骨髓中建立的。这些基质细胞系由成纤维细胞、内皮细胞和前脂肪细胞组成。我们发现这些基质细胞系根据T抗原的失活和生长条件表现出表型变化；一个前脂肪细胞系被诱导分化为脂肪细胞和成骨细胞，几个前脂肪细胞和内皮细胞系被诱导分化为肌肉细胞和脂肪细胞。一些细胞系表现出双潜能特性。这些结果表明，构成骨髓造血微环境的基质细胞源自多能间充质干细胞。
