Heberden C, Mercier T, Gaillard-Sanchez I, Huet B, Martel P
Laboratoire de Nutrition et Sécurité Alimentaire, INRA-CRJ, Jouy-en-Josas, France.
Exp Cell Res. 1995 Jun;218(2):573-6. doi: 10.1006/excr.1995.1193.
We assessed the presence and the role of membrane TGF-alpha in two rat liver epithelial cell lines, either parental or transfected with c-fos proto-oncogene. c-fos overexpressing cells had more TGF-alpha-like activity in their membranes. When TGF-alpha was removed by elastase or neutralized, the growth rates of both cell lines were markedly reduced, but to a higher extent for parental cells. If membrane TGF-alpha seemed to play a key contribution in normal cell growth, both cell lines were unable to react to the addition of soluble TGF-alpha, showing that these two forms of growth factors are not equivalent.
我们评估了膜转化生长因子α（TGF-α）在两种大鼠肝上皮细胞系中的存在情况及其作用，这两种细胞系一种是亲本细胞系，另一种是转染了c-fos原癌基因的细胞系。过表达c-fos的细胞其细胞膜具有更强的TGF-α样活性。当用弹性蛋白酶去除或中和TGF-α后，两种细胞系的生长速率均显著降低，但亲本细胞系降低的程度更高。如果膜TGF-α似乎在正常细胞生长中起关键作用，那么这两种细胞系均无法对添加的可溶性TGF-α作出反应，这表明这两种形式的生长因子并不等同。
