Aiko S, Grisham M B
Department of Physiology and Biophysics, Louisiana State University Medical Center, Shreveport, USA.
Gastroenterology. 1995 Jul;109(1):142-50. doi: 10.1016/0016-5085(95)90279-1.
BACKGROUND & AIMS: It has been reported that transgenic rats expressing the HLA-B27 and the beta 2- microglobulin genes develop spontaneous gastrointestinal (GI) inflammation; however, no systematic or quantitative evaluation of this GI inflammation has been reported. Therefore, the objective of this study was to characterize quantitatively the GI injury and inflammation observed in commercially available HLA-B27 transgenic rats.
HLA-B27 rats and Fisher 344 male controls were used for these studies. Gastric, ileal, and colonic blood-to-lumen clearances of 51Cr-ethylenediaminetetraacetic acid, tissue myeloperoxidase activities, and wet/dry ratios of the various tissues as well as plasma nitrate and nitrite levels were quantified for each control and transgenic animal.
Spontaneous ileitis and colitis developed in 5 of 10 HLA-B27 transgenic rats beginning at approximately 17 weeks of age and persisting for an additional 13 weeks. Increases in mucosal permeability and myeloperoxidase activities as well as histological analysis showed intestinal injury and chronic inflammation. Plasma levels of nitrate and nitrite, the stable decomposition products of nitric oxide, were found to be significantly enhanced (fourfold) only in those rats that developed the intestinal inflammation.
The chronic ileitis and colitis observed in HLA-B27 transgenic rats seems to be associated with enhanced NO metabolism.
据报道，表达HLA - B27和β2 - 微球蛋白基因的转基因大鼠会自发出现胃肠道（GI）炎症；然而，尚未有关于这种胃肠道炎症的系统或定量评估的报道。因此，本研究的目的是对市售HLA - B27转基因大鼠中观察到的胃肠道损伤和炎症进行定量表征。
本研究使用HLA - B27大鼠和Fisher 344雄性对照。对每只对照和转基因动物的胃、回肠和结肠的51Cr - 乙二胺四乙酸血腔清除率、组织髓过氧化物酶活性、各组织的湿/干比以及血浆硝酸盐和亚硝酸盐水平进行定量分析。
10只HLA - B27转基因大鼠中有5只在大约17周龄时开始出现自发性回肠炎和结肠炎，并持续另外13周。黏膜通透性和髓过氧化物酶活性增加以及组织学分析显示存在肠道损伤和慢性炎症。仅在那些出现肠道炎症的大鼠中，发现一氧化氮的稳定分解产物血浆硝酸盐和亚硝酸盐水平显著升高（四倍）。
在HLA - B27转基因大鼠中观察到的慢性回肠炎和结肠炎似乎与一氧化氮代谢增强有关。
