Sikurová L, Franková R
Department of Biophysics, Comenius University, Bratislava, Slovakia.
Gen Physiol Biophys. 1994 Oct;13(5):393-403.
Absorption and fluorescence emission spectroscopy was applied to study the changes in albumin modified incorporation of merocyanine 540 into liposomes composed of different lecithins (DMPC, DPPC, POPC, and egg PC). Our results confirmed high affinity of merocyanine molecules toward albumin and revealed that albumin competed with all phospholipids used for binding merocyanine 540 molecules. However, the extent of this competition was determined by the kind of phospholipid. Albumin competed very successfully with lecithins containing saturated fatty acid chains (DPPC, DMPC) and weakly with unsaturated lecithins (POPC, egg PC) for binding merocyanine 540 molecules.
采用吸收光谱和荧光发射光谱法研究了白蛋白修饰下花菁540掺入由不同卵磷脂（二肉豆蔻酰磷脂酰胆碱、二棕榈酰磷脂酰胆碱、1-棕榈酰-2-油酰磷脂酰胆碱和蛋黄卵磷脂）组成的脂质体中的变化。我们的结果证实了花菁分子对白蛋白具有高亲和力，并表明白蛋白与所有用于结合花菁540分子的磷脂存在竞争。然而，这种竞争的程度取决于磷脂的种类。在结合花菁540分子方面，白蛋白与含有饱和脂肪酸链的卵磷脂（二棕榈酰磷脂酰胆碱、二肉豆蔻酰磷脂酰胆碱）竞争非常成功，而与不饱和卵磷脂（1-棕榈酰-2-油酰磷脂酰胆碱、蛋黄卵磷脂）竞争较弱。
