Department of Biophysics MFF, Comenius University, Bratislava, Slovakia.
J Fluoresc. 1993 Dec;3(4):261-3. doi: 10.1007/BF00865275.
The interaction of the fluorescent photosensitizer merocyanine 540 (MC-540) with model phospholipid membranes was studied. Two different-colored species (monomers and dimers) of MC-540 were registered in phospholipid liposomes. Variations in both phospholipid composition (DMPC, DPPC, POPC, egg PC) and temperature (15-60°C) resulted in changes in the MC-540 monomerdimer distribution. The values of the monomer-dimer equilibrium constant of MC-540 in egg PC (K=14.8 μM), in POPC (K=26.7 μM), and in DMPC (K=271.0 μM) were determined at the temperature of 23±2°C. Suppression of MC-540 association with phospholipid bilayers was provoked by the addition of albumin to a liposome suspension. Albumin was observed to compete very successfully with lecithins containing saturated fatty acid chains (DPPC, DMPC), while only a weak competition of albumin with unsaturated lecithins (POPC, egg PC) for binding MC-540 molecules was registered.
研究了荧光光敏剂甲川花青 540（MC-540）与模型磷脂膜的相互作用。两种不同颜色的 MC-540 物种（单体和二聚体）在磷脂脂质体中被记录下来。磷脂组成（DMPC、DPPC、POPC、卵 PC）和温度（15-60°C）的变化导致 MC-540 单体-二聚体分布的变化。在 23±2°C 的温度下，确定了 MC-540 在卵 PC（K=14.8 μM）、POPC（K=26.7 μM）和 DMPC（K=271.0 μM）中的单体-二聚体平衡常数的值。向脂质体悬浮液中添加白蛋白会抑制 MC-540 与磷脂双层的结合。白蛋白与含有饱和脂肪酸链的卵磷脂（DPPC、DMPC）竞争非常成功，而白蛋白与不饱和卵磷脂（POPC、卵 PC）的结合对 MC-540 分子的竞争则较弱。
