Lobigs M, Müllbacher A
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
Immunol Cell Biol. 1995 Apr;73(2):181-4. doi: 10.1038/icb.1995.29.
Transporter associated with antigen presentation (TAP) molecules supply small peptides to the lumen of the endoplasmic reticulum for assembly with MHC class I glycoproteins. Here we report that a Syrian hamster cell line, BHK, which is commonly used in cell biological and virological investigations, is deficient in antigen presentation in a xenogeneic model. Thus when the mouse MHC class I restriction element, Kd, is expressed in BHK cells via a vaccinia virus (VV) recombinant, no significant recognition by VV-immune and Kd-restricted cytotoxic T cells is induced. This defect is apparently a consequence of a deficiency in TAP-dependent peptide transport and can be repaired when rat TAP1 and TAP2 are provided by recombinant VV expression.
抗原呈递相关转运体（TAP）分子将小肽供应至内质网腔，以便与MHC I类糖蛋白组装。我们在此报告，叙利亚仓鼠细胞系BHK常用于细胞生物学和病毒学研究，在异种模型中存在抗原呈递缺陷。因此，当小鼠MHC I类限制元件Kd通过痘苗病毒（VV）重组体在BHK细胞中表达时，不会诱导VV免疫和Kd限制的细胞毒性T细胞产生明显识别。此缺陷显然是TAP依赖性肽转运缺陷的结果，当通过重组VV表达提供大鼠TAP1和TAP2时可得到修复。
