Kamwendo S P, Musisi F L, Trees A J, Molyneux D H
Central Veterinary Laboratory, Lilongwe, Malawi.
Int J Parasitol. 1995 Jan;25(1):29-35. doi: 10.1016/0020-7519(94)00072-v.
We have previously described the presence of haemagglutinins in tissues of the tick, Rhipicephalus appendiculatus and determined their sugar specificities by inhibition experiments. In this study, haemagglutination inhibitory sugars are shown to have an effect in vivo on the abundance of Theileria parva infected salivary gland acini in Rhipicephalus appendiculatus. A significant increase (P < 0.05) was observed in T. parva acinar infection rates in the salivary glands of R. appendiculatus fed on ears of rabbits infused with melibiose and raffinose. In contrast, mannose and turanose (non-haemagglutination inhibitory sugars) did not cause elevation of T. parva acinar infection rates. The effect of melibiose in elevating acinar infections was observed when used only during T. parva maturation in the salivary glands but not during parasite pick-up from an infected bovine host. Stabilates produced from ticks with elevated acinar infections did not differ from control stabilates in infectivity to cattle, by comparison of prepatent periods to pyrexia, or parasitosis, or in the severity of reactions.
我们之前曾描述过在微小牛蜱组织中存在血凝素，并通过抑制实验确定了它们的糖特异性。在本研究中，血凝抑制糖被证明在体内对微小牛蜱中感染泰勒虫的唾液腺腺泡丰度有影响。在用注入蜜二糖和棉子糖的兔耳喂养的微小牛蜱唾液腺中，观察到泰勒虫腺泡感染率显著增加（P < 0.05）。相比之下，甘露糖和松二糖（非血凝抑制糖）并未导致泰勒虫腺泡感染率升高。仅在泰勒虫在唾液腺中成熟期间而非从感染牛宿主摄取寄生虫期间使用蜜二糖时，观察到蜜二糖对提高腺泡感染率的作用。通过比较潜伏期至发热、寄生虫病或反应严重程度，腺泡感染率升高的蜱所产生的稳定株与对照稳定株对牛的感染性没有差异。
