van Kampen G P, Korver G H, van de Stadt R J
Jan van Breemen Instituut, Centre for Rheumatology and Rehabilitation, Amsterdam, The Netherlands.
Int J Tissue React. 1994;16(4):171-9.
The anatomically intact articular cartilage (area approximately 2.5 cm2) of 6-month-old bovine sesamoid bones was cyclically (0.3 Hz) loaded with 0, 5, 25 and 50 kg in vitro for 7 days. The amount and composition of the proteoglycans that were synthesized during the last 17 hours of incubation under each regime were studied. Loads of all magnitudes produced a 45% overall increase in synthesis, which included a disproportionate increase in the synthesis of small proteoglycans. Loading with either 25 or 50 kg induced the synthesis of much larger glycosaminoglycan side-chains and a species of small proteoglycan rich in keratan sulfate. Under these greater loads, synthesis in the deep layer of the cartilage was reduced and there was focal damage to the cartilage surface.
对6月龄牛籽骨解剖结构完整的关节软骨（面积约2.5平方厘米）在体外以0、5、25和50千克的重量进行0.3赫兹的循环加载，持续7天。研究了在每种加载条件下孵育的最后17小时内合成的蛋白聚糖的数量和组成。所有强度的加载均使合成总量增加了45%，其中小蛋白聚糖的合成增加比例不成比例。加载25千克或50千克会诱导合成大得多的糖胺聚糖侧链以及一种富含硫酸角质素的小蛋白聚糖。在这些更大的负荷下，软骨深层的合成减少，软骨表面出现局灶性损伤。
