在体感刺激期间同时测量软脑膜小动脉直径和激光多普勒血流。

Simultaneous measurements of pial arteriolar diameter and laser-Doppler flow during somatosensory stimulation.

作者信息

Ngai A C, Meno J R, Winn H R

机构信息

Department of Neurological Surgery, University of Washington School of Medicine, Seattle 98104.

出版信息

J Cereb Blood Flow Metab. 1995 Jan;15(1):124-7. doi: 10.1038/jcbfm.1995.14.

DOI:10.1038/jcbfm.1995.14

PMID:7798330

Abstract

We simultaneously measured pial arteriolar diameter and changes in cortical blood flow during activation of the somatosensory cortex by sciatic nerve stimulation. The pial vasculature was visualized with a closed-cranial window technique in chloralose-anesthetized rats (n = 13). Local blood flow was monitored with laser-Doppler flowmetry. During stimulation of the sciatic nerve (0.2 V, 5 Hz, 20 s), vascular diameter and laser-Doppler flow consistently displayed similar response profiles. With 0.5-ms stimulation pulses, the responses showed an initial peak followed by a smaller but sustained plateau dilation. In contrast, 5-ms pulses evoked a monotonically rising response. Our results support the concept that pial arteriolar diameter changes reflect cortical blood flow responses during somatosensory stimulation.

摘要

我们在通过坐骨神经刺激激活体感皮层期间，同时测量软脑膜小动脉直径和皮层血流变化。在水合氯醛麻醉的大鼠（n = 13）中，采用闭合颅骨开窗技术观察软脑膜血管系统。用激光多普勒血流仪监测局部血流。在坐骨神经刺激（0.2 V，5 Hz，20 s）期间，血管直径和激光多普勒血流始终显示出相似的反应曲线。使用0.5毫秒的刺激脉冲时，反应显示出一个初始峰值，随后是较小但持续的平台期扩张。相比之下，5毫秒的脉冲引起单调上升的反应。我们的结果支持这样的概念，即软脑膜小动脉直径变化反映体感刺激期间的皮层血流反应。