低甲基化转运RNA不支持噬菌体RNA指导的体外蛋白质合成。

Undermethylated transfer RNA does not support phage RNA-directed in vitro protein synthesis.

作者信息

Stulberg M P, Sutton M, Isham K R

出版信息

Biochim Biophys Acta. 1976 Jul 2;435(3):251-7. doi: 10.1016/0005-2787(76)90106-4.

Abstract

A cell-free protein synthesis system from Escherichia coli Q13 was depleted of mRNA and tRNA so that restoration of maximum activity was dependent of the addition of these components. Protein synthesis, directed by either MS2 or Qbeta phage RNA, was stimulated significantly by the addition of normal tRNA from either E. coli Q13 or B. In contrast, undermethylated tRNA from methionine-starved E. coli RCrel did not cause this stimulation. It is concluded that undermethylated tRNA Lacks sufficient base modifications to function in protein synthesis.

摘要

来自大肠杆菌Q13的无细胞蛋白质合成系统去除了mRNA和tRNA，因此最大活性的恢复依赖于这些组分的添加。由MS2或Qβ噬菌体RNA指导的蛋白质合成，通过添加来自大肠杆菌Q13或B的正常tRNA而得到显著刺激。相比之下，来自蛋氨酸饥饿的大肠杆菌RCrel的未充分甲基化的tRNA并未引起这种刺激。得出的结论是，未充分甲基化的tRNA缺乏足够的碱基修饰以在蛋白质合成中发挥作用。