Morita K, Maemoto T, Honda S, Onishi K, Murata M, Tanaka M, Igarashi A
Department of Virology, Nagasaki University, Japan.
J Med Virol. 1994 Sep;44(1):54-8. doi: 10.1002/jmv.1890440111.
Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis of dengue infection.
来自输入性登革热和登革出血热患者的血清标本未经任何RNA纯化，直接进行逆转录和聚合酶链反应（RT-PCR）。在3小时内检测到扩增的病毒基因组。PCR结果与使用培养的蚊细胞进行病毒分离的结果密切相符，表明直接RT-PCR程序极大地促进了登革热感染的快速诊断。
