通过向犬类关节腔内注射犬单核细胞条件培养基，诱导犬滑液（SF）中蛋白聚糖片段水平升高，以及软骨、滑膜和SF中基质溶解素水平升高。
Induction of increased levels of proteoglycan fragments in synovial fluid (SF) and increased levels of stromelysin in cartilage, synovium and SF by intraarticular injection of canine monocyte conditioned medium into dogs.
作者信息
Lark M W, MacNaul K A, Donatelli S, McDonnell J, Hoerrner L A, Stevens K A, Saphos C A, Bayne E K, Hutchinson N I, Moore V L
机构信息
Department of Immunology, Merck Research Laboratories, Rahway, NJ 07065.
出版信息
J Rheumatol. 1994 Sep;21(9):1716-24.
OBJECTIVE
To study the effects of the intraarticular injection of canine monocyte conditioned medium (cMCM) into dogs on proteoglycan fragment and stromelysin levels in the joint.
METHODS
cMCM was injected intraarticularly into dogs, and the levels of proteoglycan fragments in synovial fluid (SF) as well as stromelysin levels in cartilage, synovium, and SF were assessed after 12 h.
RESULTS
There was a 4-fold increase of proteoglycan fragment levels and a 6-fold increase in stromelysin levels in SF, and a 4.4-fold increase in stromelysin levels in cartilage extracts. Elevated mRNA levels were detected in both synovium and cartilage. By immunofluorescence staining, stromelysin was localized in chondrocytes throughout the cartilage and in synovial cells.
CONCLUSION
Intraarticular injection of cMCM stimulated the expression of stromelysin mRNA and protein in cartilage and synovium and caused marked increases in stromelysin protein and proteoglycan fragment levels in SF.
目的
研究向犬关节内注射犬单核细胞条件培养基（cMCM）对关节中蛋白聚糖片段和基质溶解素水平的影响。
方法
将cMCM关节内注射到犬体内，12小时后评估滑液（SF）中蛋白聚糖片段的水平以及软骨、滑膜和SF中基质溶解素的水平。
结果
SF中蛋白聚糖片段水平增加了4倍，基质溶解素水平增加了6倍，软骨提取物中基质溶解素水平增加了4.4倍。在滑膜和软骨中均检测到mRNA水平升高。通过免疫荧光染色，基质溶解素定位于整个软骨中的软骨细胞和滑膜细胞中。
结论
关节内注射cMCM刺激了软骨和滑膜中基质溶解素mRNA和蛋白的表达，并导致SF中基质溶解素蛋白和蛋白聚糖片段水平显著增加。
Zotero 插件