Murata M, Sugimoto C, Kodama H, Onuma M
Department of Veterinary Science, National Institute of Health, Shinjuku, Tokyo, Japan.
Jpn J Vet Res. 1994 Aug;42(2):89-94.
As there are few data on primary structures of fish apolipoproteins (apo), we determined the N-terminal sequence of a 28 kDa major serum protein of rainbow trout, a possible homologue of apoAI protein in higher vertebrates. Rainbow trout serum was separated by two-dimensional electrophoresis. A spot of the 28 kDa protein (pl 5.45) was cut out and directly applied to a protein sequencer. We succeeded in sequencing 30 amino acids from the N-terminal. The sequence of the 28 kDa protein shared a high similarity with that of apoAI of Atlantic salmon (Salmo salar). These results showed that the 28 kDa protein is apoAI of rainbow trout.
由于关于鱼类载脂蛋白（apo）一级结构的数据较少，我们测定了虹鳟鱼一种28 kDa主要血清蛋白的N端序列，它可能是高等脊椎动物中apoAI蛋白的同源物。虹鳟鱼血清通过二维电泳进行分离。切下28 kDa蛋白（等电点5.45）的一个斑点，直接应用于蛋白质测序仪。我们成功地从N端测序了30个氨基酸。28 kDa蛋白的序列与大西洋鲑（Salmo salar）的apoAI序列具有高度相似性。这些结果表明，28 kDa蛋白是虹鳟鱼的apoAI。
