Gentry W L, Critser E S
Fertility Institute, Methodist Hospital of Indiana, Indianapolis.
Obstet Gynecol. 1995 Jan;85(1):57-9. doi: 10.1016/0029-7844(94)00338-e.
To evaluate the frequency of mouse pups born following blastocyst biopsy and embryo transfer compared to nonbiopsied controls.
ICR Swiss albino and C57B1/6 mice served as embryo donors. Pregnant mare serum gonadotropin treatment was followed 46-52 hours later by hCG, when donors were paired with fertile males. Mating was confirmed the following day and embryos were collected on the afternoon of day 4. After overnight culture, hatching trophoblast was excised by micromanipulation with a fine-pulled glass pipette. Embryos (206 controls, 206 biopsied) were transferred to 26 pseudopregnant recipients. Alternate mouse strains were used to identify pups born from control or biopsied embryos.
The end point was percentage of pups born ((number born/number transferred) x 100), using angular transformation before analysis. There was no significant difference (P > .1) between percent live-born in control (27.7%) or biopsied (34.5%) embryos, nor were there any strain differences.
These data support the hypothesis that the developmental potential of murine blastocysts is not affected adversely by the biopsy procedure.
评估与未活检的对照组相比，囊胚活检及胚胎移植后出生的幼鼠频率。
ICR瑞士白化小鼠和C57B1/6小鼠作为胚胎供体。在注射孕马血清促性腺激素46 - 52小时后注射人绒毛膜促性腺激素，此时将供体与可育雄性小鼠配对。次日确认交配成功，在第4天下午收集胚胎。过夜培养后，用精细拉制的玻璃微吸管通过显微操作切除孵化的滋养层。将胚胎（206个对照组，206个活检组）移植到26只假孕受体小鼠体内。使用交替的小鼠品系来识别由对照胚胎或活检胚胎出生的幼鼠。
终点指标为出生幼鼠的百分比（（出生数量/移植数量）×100），分析前采用角度转换。对照胚胎（27.7%）和活检胚胎（34.5%）的活产百分比之间无显著差异（P > 0.1），也没有品系差异。
这些数据支持以下假设，即囊胚活检程序不会对小鼠囊胚的发育潜能产生不利影响。
