• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Expression of the E. coli fpg gene in mammalian cells reduces the mutagenicity of gamma-rays.

作者信息

Laval F

机构信息

Groupe Radiochimie de l'ADN, U 247 INSERM, Institut Gustave Roussy, Villejuif, France.

出版信息

Nucleic Acids Res. 1994 Nov 25;22(23):4943-6. doi: 10.1093/nar/22.23.4943.

DOI:10.1093/nar/22.23.4943
PMID:7800484
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC523760/
Abstract

The E. coli fpg gene encodes the formamido-pyrimidine-DNA-glycosylase (FPG protein) which specifically removes the formamido-pyrimidine and C8-oxoGuanine residues from gamma-irradiated DNA. The fpg gene was ligated in the psV2 vector and transfected into the Chinese hamster CHO and V-79 cells. The transfected cells expressed a formamido-pyrimidine-DNA-glycosylase activity 30 to 40-fold over the constitutive level. The resistance of CHO and V-79 cells to the lethal effect of gamma-rays was similar in control and transfected cells. Furthermore CHO cells expressing the fpg gene had the same resistance to the lethal effect of hydrogen peroxide as control cells. However, the sensitivity to the mutagenic effect of gamma-rays, measured as 6-thioguanine resistance, decreased both in CHO and V-79 transfected cells. Since the lethal effect of gamma-rays was not modified in cells overproducing the FPG protein, the results suggest that this protein protects the cells against the mutagenic lesions formed by ionizing radiations, and among them C8-oxoguanine.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/988f/523760/486ae7c402cb/nar00047-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/988f/523760/486ae7c402cb/nar00047-0090-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/988f/523760/486ae7c402cb/nar00047-0090-a.jpg

相似文献

1
Expression of the E. coli fpg gene in mammalian cells reduces the mutagenicity of gamma-rays.
Nucleic Acids Res. 1994 Nov 25;22(23):4943-6. doi: 10.1093/nar/22.23.4943.
2
Reduction of the toxicity and mutagenicity of aziridine in mammalian cells harboring the Escherichia coli fpg gene.在携带大肠杆菌fpg基因的哺乳动物细胞中降低氮丙啶的毒性和诱变性。
Nucleic Acids Res. 1996 May 1;24(9):1742-6. doi: 10.1093/nar/24.9.1742.
3
Increased resistance to N,N',N"-triethylenethiophosphoramide (thiotepa) in cells expressing the Escherichia coli formamidopyrimidine-DNA glycosylase.在表达大肠杆菌甲酰胺嘧啶 - DNA糖基化酶的细胞中对N,N',N"-三乙烯硫代磷酰胺（噻替派）的抗性增加。
Cancer Res. 1996 Aug 15;56(16):3721-4.
4
The role of nucleotide excision repair of Escherichia coli in repair of spontaneous and gamma-radiation-induced DNA damage in the lacZalpha gene.大肠杆菌核苷酸切除修复在lacZα基因中自发和γ辐射诱导的DNA损伤修复中的作用。
Mutat Res. 2000 Jul 25;460(2):117-25. doi: 10.1016/s0921-8777(00)00021-5.
5
Effect of single mutations on the specificity of Escherichia coli FPG protein for excision of purine lesions from DNA damaged by free radicals.单个突变对大肠杆菌FPG蛋白从自由基损伤的DNA中切除嘌呤损伤特异性的影响。
Free Radic Biol Med. 2001 Sep 15;31(6):816-23. doi: 10.1016/s0891-5849(01)00659-1.
6
Repair of imidazole ring-opened purines in DNA: overproduction of the formamidopyrimidine-DNA glycosylase of Escherichia coli using plasmids containing the fpg+ gene.DNA中咪唑环开环嘌呤的修复：利用含有fpg⁺基因的质粒过量表达大肠杆菌的甲酰胺嘧啶-DNA糖基化酶。
Ann Ist Super Sanita. 1989;25(1):27-31.
7
The influence of formamidopyrimidine-DNA glycosylase on the spontaneous and gamma-radiation-induced mutation spectrum of the lacZ alpha gene.甲酰胺嘧啶-DNA糖基化酶对lacZα基因自发及γ射线诱导突变谱的影响
Mutat Res. 1999 Oct 22;435(2):141-50. doi: 10.1016/s0921-8777(99)00043-9.
8
Retrovirus-mediated expression of the base excision repair proteins, formamidopyrimidine DNA glycosylase or human oxoguanine DNA glycosylase, protects hematopoietic cells from N,N',N"-triethylenethiophosphoramide (thioTEPA)-induced toxicity in vitro and in vivo.逆转录病毒介导的碱基切除修复蛋白、甲酰胺嘧啶DNA糖基化酶或人氧鸟嘌呤DNA糖基化酶的表达，在体外和体内均可保护造血细胞免受N,N',N"-三乙烯硫代磷酰胺（噻替派）诱导的毒性作用。
Cancer Res. 2001 Jul 1;61(13):5116-25.
9
Clustered DNA damage induced by gamma radiation in human fibroblasts (HF19), hamster (V79-4) cells and plasmid DNA is revealed as Fpg and Nth sensitive sites.在人类成纤维细胞（HF19）、仓鼠（V79-4）细胞和质粒DNA中，由γ辐射诱导产生的簇状DNA损伤表现为对Fpg和Nth敏感的位点。
Nucleic Acids Res. 2002 Aug 1;30(15):3464-72. doi: 10.1093/nar/gkf467.
10
The influence of combined Fpg- and MutY-deficiency on the spontaneous and gamma-radiation-induced mutation spectrum in the lacZalpha gene of m13mp10.Fpg和MutY联合缺陷对m13mp10的lacZα基因中自发及γ射线诱导突变谱的影响
Mutat Res. 2000 Nov 9;461(3):189-95. doi: 10.1016/s0921-8777(00)00050-1.

引用本文的文献

1
Programming of Cell Resistance to Genotoxic and Oxidative Stress.细胞对基因毒性和氧化应激的抗性编程
Biomedicines. 2018 Jan 2;6(1):5. doi: 10.3390/biomedicines6010005.
2
Accelerated repair and reduced mutagenicity of DNA damage induced by cigarette smoke in human bronchial cells transfected with E.coli formamidopyrimidine DNA glycosylase.经 E.coli 型(formamidopyrimidine DNA glycosylase) 转染的人支气管细胞中，香烟烟雾诱导的 DNA 损伤的加速修复和降低的致突变性。
PLoS One. 2014 Jan 31;9(1):e87984. doi: 10.1371/journal.pone.0087984. eCollection 2014.
3
Endogenous DNA damage clusters in human skin, 3-D model, and cultured skin cells.

本文引用的文献

1
Induction of mutations in mouse FM3A cells by treatment with riboflavin plus visible light and its possible relation with formation of 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in DNA.
Carcinogenesis. 1993 May;14(5):1069-71. doi: 10.1093/carcin/14.5.1069.
2
SOS-independent mutagenesis in lacZ induced by methylene blue plus visible light.亚甲蓝加可见光诱导的lacZ中的非SOS依赖性诱变。
Mol Gen Genet. 1993 Jan;236(2-3):433-9. doi: 10.1007/BF00277144.
3
Single-stranded shuttle phagemid for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA induces targeted G.C-->T.A transversions in simian kidney cells.用于哺乳动物细胞诱变研究的单链穿梭噬菌粒：DNA中的8-氧代鸟嘌呤在猴肾细胞中诱导靶向的G.C→T.A颠换
人类皮肤、三维模型和培养的皮肤细胞中的内源性DNA损伤簇。
Free Radic Biol Med. 2005 Sep 15;39(6):832-9. doi: 10.1016/j.freeradbiomed.2005.05.008.
4
Deficient DNA-ligase activity in the metabolic disease tyrosinemia type I.代谢性疾病I型酪氨酸血症中DNA连接酶活性不足。
Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12614-8. doi: 10.1073/pnas.95.21.12614.
5
Reduction of the toxicity and mutagenicity of aziridine in mammalian cells harboring the Escherichia coli fpg gene.在携带大肠杆菌fpg基因的哺乳动物细胞中降低氮丙啶的毒性和诱变性。
Nucleic Acids Res. 1996 May 1;24(9):1742-6. doi: 10.1093/nar/24.9.1742.
6
Overexpression of a Rrp1 transgene reduces the somatic mutation and recombination frequency induced by oxidative DNA damage in Drosophila melanogaster.Rrp1转基因的过表达降低了黑腹果蝇中由氧化性DNA损伤诱导的体细胞突变和重组频率。
Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1607-12. doi: 10.1073/pnas.93.4.1607.
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):1122-6. doi: 10.1073/pnas.90.3.1122.
4
Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in human cells.人类细胞中两种针对8-羟基鸟嘌呤（7,8-二氢-8-氧代鸟嘌呤）的DNA修复酶的证据。
J Biol Chem. 1993 Sep 15;268(26):19416-21.
5
Evidence for the accumulation of oxidative stress during cellular ageing of human diploid fibroblasts.
Biochem Biophys Res Commun. 1994 Sep 15;203(2):1063-8. doi: 10.1006/bbrc.1994.2290.
6
DNA base damage generated in vivo in hepatic chromatin of mice upon whole body gamma-irradiation.全身γ射线照射后小鼠肝脏染色质在体内产生的DNA碱基损伤。
Int J Radiat Biol. 1993 Dec;64(6):645-50. doi: 10.1080/09553009314551881.
7
Increased resistance of the Chinese hamster mutant irs1 cells to monofunctional alkylating agents by transfection of the E. coli or mammalian N3-methyladenine-DNA-glycosylase genes.通过转染大肠杆菌或哺乳动物的N3-甲基腺嘌呤-DNA-糖基化酶基因，中国仓鼠突变体irs1细胞对单功能烷化剂的抗性增强。
Mutat Res. 1993 Mar;293(3):187-95. doi: 10.1016/0921-8777(93)90069-s.
8
Two rotameric forms of open ring 7-methylguanine are present in alkylated polynucleotides.在烷基化多核苷酸中存在开环7-甲基鸟嘌呤的两种旋转异构体形式。
Nucleic Acids Res. 1984 Jul 11;12(13):5429-39. doi: 10.1093/nar/12.13.5429.
9
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Formation of 8-hydroxyguanine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair.产生活性氧的试剂在细胞DNA中形成8-羟基鸟嘌呤部分及其修复证据。
Carcinogenesis. 1986 Nov;7(11):1849-51. doi: 10.1093/carcin/7.11.1849.