Tsagarakis C, Marchal T, Magnol J P, Fournel C, Dezutter-Dambuyant C, Schmitt D
Ecole Nationale Vétérinaire de Lyon, Laboratoire d'Immunopathologie, Marcy l'Etoile, France.
Res Virol. 1994 May-Aug;145(3-4):245-9. doi: 10.1016/s0923-2516(07)80029-x.
In order to identify an equivalent of Langerhans cells in cat stratified epithelia, we used a panel of monoclonal antibodies known to be reactive with membrane antigens present on human Langerhans cells. The labelling was carried out by immunoperoxidase staining, for examination by light microscope, and by immunogold labelling, for electron microscopy. Out of 18 antibodies tested, only one, MHM23 antibody, specific against CD18 antigen, presented reactivity with dendritic epithelial cells on either frozen sections, epidermal sheets or cell suspension cytospins. On the ultrastructural level, these clear, dendritic, CD18+ cells showed "zipper-like" shapes similar to Birbeck granules, which are characteristic ultrastructural markers of Langerhans cells. This observation favours the hypothesis that these CD18+ cells in cat stratified epithelia are the equivalent of human Langerhans cells. These labelled cells were found in all epidermal locations and in the mucous membranes (oral, vaginal, rectal and oesophageal membranes). As feline immunodeficiency virus (FIV) transmission may occur through these membranes, the involvement of these feline Langerhans cells was studied in cats seropositive for FIV.
为了在猫的复层上皮中鉴定出与朗格汉斯细胞等同的细胞，我们使用了一组已知可与人朗格汉斯细胞上存在的膜抗原发生反应的单克隆抗体。通过免疫过氧化物酶染色进行标记，用于光学显微镜检查，以及通过免疫金标记进行标记，用于电子显微镜检查。在测试的18种抗体中，只有一种针对CD18抗原的MHM23抗体，在冰冻切片、表皮片或细胞悬液涂片上与树突状上皮细胞呈现反应性。在超微结构水平上，这些清晰的、树突状的、CD18阳性细胞呈现出类似于伯贝克颗粒的“拉链样”形状，伯贝克颗粒是朗格汉斯细胞的特征性超微结构标志物。这一观察结果支持了这样一种假说，即猫复层上皮中的这些CD18阳性细胞等同于人类朗格汉斯细胞。这些标记细胞在所有表皮部位以及黏膜（口腔、阴道、直肠和食管黏膜）中均有发现。由于猫免疫缺陷病毒（FIV）可能通过这些黏膜传播，因此对FIV血清阳性猫中这些猫朗格汉斯细胞的参与情况进行了研究。
