Peters M, Hewicker-Trautwein M
Institute of Microbiology and Infectious Diseases of Animals, School of Veterinary Medicine, Hannover, Germany.
Vet Microbiol. 1994 Jul;41(1-2):19-28. doi: 10.1016/0378-1135(94)90132-5.
The uptake of Listeria monocytogenes by different brain cells was studied in primary dissociated brain cell cultures derived from murine fetuses. In respect to the supposed intraaxonal migration of Listeria monocytogenes in the pathogenesis of listeric focal brain stem encephalitis, it was examined whether the bacterium was internalized by neurons. Infection rates of distinct cell types were determined by double immunofluorescence with antibodies against cell type-specific markers and the bacterial pathogen. Because of the changing composition of the cultures and time-dependent expression of the oligodendrocyte marker galactocerebroside (GC), infections were carried out on day 4, 6, 8, and 15 in vitro. Listeria monocytogenes was detected predominantly within macrophages. Astrocytes, oligodendrocytes, and fibronectin-expressing cells were infected to a lesser extent. The lowest rates of infection were observed in neurons. A tropism of Listeria monocytogenes for neurons was not detected in vitro.
在源自小鼠胎儿的原代解离脑细胞培养物中，研究了不同脑细胞对单核细胞增生李斯特菌的摄取情况。关于单核细胞增生李斯特菌在李斯特菌性局灶性脑干脑炎发病机制中假定的轴突内迁移，研究了该细菌是否被神经元内化。通过针对细胞类型特异性标志物和细菌病原体的抗体进行双重免疫荧光测定不同细胞类型的感染率。由于培养物组成的变化以及少突胶质细胞标志物半乳糖脑苷脂（GC）的时间依赖性表达，在体外第4、6、8和15天进行感染。单核细胞增生李斯特菌主要在巨噬细胞内被检测到。星形胶质细胞、少突胶质细胞和表达纤连蛋白的细胞感染程度较低。在神经元中观察到的感染率最低。在体外未检测到单核细胞增生李斯特菌对神经元的嗜性。
