Harada T, Saito H, Kouhara H, Kurebayashi S, Kasayama S, Terakawa N, Kishimoto T, Sato B
Department of Obstetrics and Gynecology, Tottori University Medical School, Japan.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1057-63. doi: 10.1006/bbrc.1994.2773.
The arrangement of exons and introns encoding 5'-side of murine fibroblast growth factor (FGF) receptor 1 (FGFR-1) gene was mapped. A large intron with a size of 14 kb was identified between exon 1 and exon 2. In addition, all FGFR-1 subtypes including a unique variant form with 12 amino acids insertion and two amino acids deletion were observed to be able to be generated through alternative splicing. Furthermore, complete sequencing of the 5'-region of FGFR-1 mRNA revealed that a relatively large open reading frame precedes the major open reading frame encoding FGFR-1. These results indicate that FGFR-1 mRNAs are uniquely translated from an internal translation start site.
对编码小鼠成纤维细胞生长因子（FGF）受体1（FGFR-1）基因5'端的外显子和内含子排列进行了定位。在外显子1和外显子2之间鉴定出一个大小为14 kb的大内含子。此外，观察到所有FGFR-1亚型，包括具有12个氨基酸插入和2个氨基酸缺失的独特变体形式，都能够通过可变剪接产生。此外，FGFR-1 mRNA 5'区域的完整测序显示，在编码FGFR-1的主要开放阅读框之前有一个相对较大的开放阅读框。这些结果表明，FGFR-1 mRNA是从内部翻译起始位点独特翻译而来的。
