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Functional reconstitution of Ehrlich cell plasma membrane ferricyanide reductase.

作者信息

Medina M A, Núñez de Castro I

机构信息

Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.

出版信息

Biochem Biophys Res Commun. 1994 Dec 15;205(2):1109-13. doi: 10.1006/bbrc.1994.2780.

DOI:10.1006/bbrc.1994.2780
PMID:7802639
Abstract

Native plasma membrane fractions isolated from Ehrlich ascites tumor cells were used as the starting point for two easy and efficient procedures to functionally reconstitute ferricyanide reductase activity. The obtained proteoliposomes showed high specific activities. The reconstituted ferricyanide reductase seemed to have one or more cysteine essential residues, as judged by inhibition with sulfhydryl reagents.

摘要

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