Medina M A, Núñez de Castro I
Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1109-13. doi: 10.1006/bbrc.1994.2780.
Native plasma membrane fractions isolated from Ehrlich ascites tumor cells were used as the starting point for two easy and efficient procedures to functionally reconstitute ferricyanide reductase activity. The obtained proteoliposomes showed high specific activities. The reconstituted ferricyanide reductase seemed to have one or more cysteine essential residues, as judged by inhibition with sulfhydryl reagents.