Inui H, Kondo T, Konishi F, Kitami Y, Inagami T
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1338-44. doi: 10.1006/bbrc.1994.2812.
In rat vascular smooth muscle cells, platelet-derived growth factor (PDGF), stimulated phosphatidic acid synthesis by activating both of the two alternative pathways, diacyglycerol kinase (DGK) and phospholipase D (PLD). Genistein, a tyrosine kinase inhibitor, inhibited PLD activation but not DGK activation, the latter was inhibited selectively by R 59022. PDGF-induced DNA synthesis was partially inhibited by genistein or R 59022, but these inhibitors had no effect on phorbol ester-induced DNA synthesis. Further, the specific effects of these inhibitors on PDGF-induced DNA synthesis were additive.
