Akintunde A, Eisenman L M
Department of Anatomy and Developmental Biology, Thomas Jefferson University, Philadelphia, PA 19107.
J Chem Neuroanat. 1994 Jul;7(1-2):75-86. doi: 10.1016/0891-0618(94)90009-4.
The parasagittal parcellation of the mammalian cerebellar cortex has been revealed using anatomical, electrophysiological, histological and immunological techniques. Correlation studies have been carried out to determine whether a common organizational plan encompassing the various afferent, efferent and intrinsic maps may exist in the mammalian cerebellum. Many of these studies utilized the parasagittal Purkinje cell antigenic banding pattern as revealed by the monoclonal antibody against zebrin antigens as a standard reference. In this study, the pattern of labelled mossy fiber terminals originating from the external cuneate nucleus was determined and compared with the Purkinje cell antigenic zebrin bands in the same sections. External cuneocerebellar fibers in the mouse were observed to project in well-delineated parasagittal terminal distribution zones, primarily to the ipsilateral vermal cerebellar cortex. However, there was a very minor, but consistent contralateral component. The external cuneocerebellar fiber termination pattern in the mouse differed from that seen in other rodents such as the rat. Comparison of the external cuneucerebellar terminal zones in sections immunohistochemically stained for the zebrin II antigen revealed that the boundaries of the terminal fields of the external cuneocerebellar projection do not always align with those of the zebrin II antigenic bands. These results strongly suggest that mossy fibers have a complicated relationship to zebrin defined compartments. Therefore, the designation 'functional unit' of the cerebellum or 'cerebellar module' remains uncertain at this time.
利用解剖学、电生理学、组织学和免疫学技术，已揭示了哺乳动物小脑皮质的矢旁分区。已经开展了相关性研究，以确定在哺乳动物小脑中是否可能存在一种包含各种传入、传出和固有图谱的共同组织模式。这些研究中有许多利用了抗zebrin抗原的单克隆抗体所揭示的矢旁浦肯野细胞抗原带模式作为标准参考。在本研究中，确定了源自外侧楔状核的标记苔藓纤维终末的模式，并将其与同一切片中的浦肯野细胞抗原性zebrin带进行比较。观察到小鼠的外侧楔小脑纤维投射到界限清晰的矢旁终末分布区，主要投射到同侧蚓部小脑皮质。然而，存在非常小但一致的对侧成分。小鼠的外侧楔小脑纤维终末模式与其他啮齿动物如大鼠中所见的不同。对用zebrin II抗原进行免疫组织化学染色的切片中的外侧楔小脑终末区进行比较发现，外侧楔小脑投射的终末野边界并不总是与zebrin II抗原带的边界对齐。这些结果强烈表明苔藓纤维与zebrin定义的区室有复杂的关系。因此，小脑的“功能单位”或“小脑模块”这一命名目前仍不确定。
