Antal M, Polgár E, Berki A, Birinyi A, Puskár Z
Department of Anatomy, University Medical School, Debrecen, Hungary.
Eur J Morphol. 1994 Aug;32(2-4):201-6.
The development, morphological and neurochemical properties of specific populations of interneurons were investigated in the ventral horn of the embryonic and mature chick lumbosacral spinal cord by using pre- and post-embedding immunocytochemical as well as anterograde axonal tracing techniques. We have identified and traced the morphological maturation of the following cell groups: (1) Neurons immunoreactive for calbindin-D 28k (CaB), a calcium-binding protein that has been reported to be a marker of certain subsets of excitatory spinal neurons. We have distinguished and traced the maturation of three CaB-immunoreactive cell groups in the ventral horn; (2) Neurons immunoreactive for GABA and glycine, the two putative inhibitory amino acid neurotransmitters in the spinal cord; (3) Neurons within the nucleus marginalis, a cell group located in the ventrolateral aspect of the white matter in close proximity to the lateral motor column. The characteristic features of the development of these neurons are discussed and correlated with previous neuroanatomical and physiological studies concerning motor functions in the developing chick spinal cord.
利用包埋前和包埋后免疫细胞化学以及顺行轴突追踪技术，研究了胚胎期和成熟期鸡腰骶脊髓腹角中特定中间神经元群的发育、形态和神经化学特性。我们已经识别并追踪了以下细胞群的形态成熟过程：(1) 对钙结合蛋白-D 28k（CaB）免疫反应的神经元，钙结合蛋白-D 28k是一种钙结合蛋白，据报道是某些兴奋性脊髓神经元亚群的标志物。我们已经区分并追踪了腹角中三个CaB免疫反应细胞群的成熟过程；(2) 对γ-氨基丁酸（GABA）和甘氨酸免疫反应的神经元，这两种是脊髓中假定的抑制性氨基酸神经递质；(3) 边缘核内的神经元，这是位于白质腹外侧、紧邻外侧运动柱的一个细胞群。讨论了这些神经元发育的特征，并将其与先前关于鸡脊髓发育过程中运动功能的神经解剖学和生理学研究相关联。
