González A, Muñoz M, Muñoz A, Marin O, Smeets W J
Departamento de Biologia Celular, Facultad de Biologia, Universidad Complutense, Madrid, Spain.
Eur J Morphol. 1994 Aug;32(2-4):271-4.
Recent studies have revealed a rather dense dopaminergic innervation of basal forebrain areas in anurans and urodeles. The aim of the present study is not only to extend these observations to the third order of amphibians, i.e. the Gymnophiona, but also to determine the cells of origin of the dopaminergic projection to the basal forebrain by means of combined tract-tracing/transmitter-immunohistochemical techniques. Immunohistochemistry with antibodies against tyrosine hydroxylase (TH) and dopamine (DA) demonstrated some remarkable differences in the distribution of DA fibers within the basal forebrain. Whereas in anurans the nucleus accumbens receives a major DA input, in urodeles the DA input to the striatum prevails. In gymnophionans, both basal forebrain structures receive an equally dense DA innervation. The double-labeling studies revealed also differences in the distribution of the cells of origin of the DA striatal input. Although in anurans and urodeles DA cells in both the posterior tubercle and the midbrain tegmentum contribute to this projection, the mesostriatal projection of urodeles is more elaborated than that of anurans.
最近的研究表明，无尾两栖类和有尾两栖类动物的基底前脑区域存在相当密集的多巴胺能神经支配。本研究的目的不仅是将这些观察结果扩展到两栖纲的第三目，即蚓螈目，还要通过联合束路追踪/递质免疫组织化学技术确定投射到基底前脑的多巴胺能神经元的起源细胞。用抗酪氨酸羟化酶（TH）和多巴胺（DA）的抗体进行免疫组织化学研究，结果显示基底前脑内DA纤维的分布存在一些显著差异。在无尾两栖类动物中，伏隔核接受主要的DA输入，而在有尾两栖类动物中，纹状体的DA输入占主导地位。在蚓螈目动物中，这两个基底前脑结构接受的DA神经支配同样密集。双标记研究还揭示了DA纹状体输入的起源细胞分布存在差异。尽管在无尾两栖类和有尾两栖类动物中，后结节和中脑被盖中的DA细胞都参与了这一投射，但有尾两栖类动物的中脑纹状体投射比无尾两栖类动物的更精细。
