Mizutani Y, Bonavida B
Department of Microbiology and Immunology, UCLA School of Medicine, University of California at Los Angeles 90024.
Biotherapy. 1993;7(2):109-14. doi: 10.1007/BF01877734.
The OVC-8 human ovarian cancer cell line constitutively expresses tumor necrosis factor alpha (TNF-alpha) mRNA and protein and is resistant to TNF-alpha. When OVC-8 cells are treated with pentoxifylline (PTX), the level of mRNA for TNF-alpha is markedly reduced. Combination treatment of OVC-8 cells with PTX and TNF-alpha overcomes the resistance. PTX-treatment has no effect on the expression of TNF-alpha mRNA in C30 cells, which do not constitutively express TNF-alpha mRNA. The combination of PTX and TNF-alpha do not overcome the resistance of C30 cells to TNF-alpha. PTX or anti-TNF-alpha monoclonal antibody has no effect on the growth of OVC-8 cells, suggesting that the growth of OVC-8 cells does not depend upon an autocrine action of TNF-alpha. The synergistic cytotoxic effect obtained with ovarian cancer cells suggests that the combination of PTX and TNF-alpha could be applied clinically in the therapy of TNF-alpha-producing ovarian cancer.
OVC - 8人卵巢癌细胞系组成性表达肿瘤坏死因子α（TNF -α）的mRNA和蛋白，并且对TNF -α具有抗性。当用己酮可可碱（PTX）处理OVC - 8细胞时，TNF -α的mRNA水平显著降低。PTX与TNF -α联合处理可克服这种抗性。PTX处理对C30细胞中TNF -αmRNA的表达没有影响，C30细胞不组成性表达TNF -αmRNA。PTX和TNF -α的联合处理不能克服C30细胞对TNF -α的抗性。PTX或抗TNF -α单克隆抗体对OVC - 8细胞的生长没有影响，这表明OVC - 8细胞的生长不依赖于TNF -α的自分泌作用。卵巢癌细胞获得的协同细胞毒性作用表明，PTX和TNF -α的联合应用可在临床上用于治疗产生TNF -α的卵巢癌。
