纳菲ion修饰碳糊电极在药物制剂和生物流体中沙丁胺醇吸附溶出伏安测定中的应用。

Application of a Nafion-modified carbon paste electrode for the absorptive stripping voltammetric determination of fenoterol in pharmaceutical preparations and biological fluids.

作者信息

Boyd D, Barreira Rodríguez J R, Tuñón Blanco P, Smyth M R

机构信息

Department of Physical and Analytical Chemistry, University of Oviedo, Asturias, Spain.

出版信息

J Pharm Biomed Anal. 1994 Sep;12(9):1069-74. doi: 10.1016/0731-7085(94)00055-7.

DOI:10.1016/0731-7085(94)00055-7

PMID:7803553

Abstract

The preconcentration of fenoterol on a Nafion-modified carbon paste electrode and its subsequent determination using differential pulse voltammetry is described. The effect of pH and percentage Nafion concentration on the accumulation behaviour of fenoterol was studied, and accumulation curves, calibration graphs and reproducibility studies at two different Nafion concentrations have been carried out in the range 2.5 x 10(-8)-5.0 x 10(-7) M fenoterol. A limit of detection in aqueous solutions, calculated using a signal-to-noise ratio (S/N) of 3, was 9.0 x 10(-9) M. Application of the electrode to pharmaceutical preparations, without sample pretreatment, resulted in acceptable deviation from the stated concentration (RSD = +/- 3.81%, n = 4). For more complex matrices, a suitable extraction procedure was developed, resulting in recoveries of > 90% (urine) and > 75% (serum).

摘要

本文描述了非诺特罗在Nafion修饰碳糊电极上的预富集及其随后使用差分脉冲伏安法进行的测定。研究了pH值和Nafion浓度百分比对非诺特罗富集行为的影响，并在2.5×10⁻⁸ - 5.0×10⁻⁷ M非诺特罗范围内，针对两种不同的Nafion浓度进行了富集曲线、校准曲线和重现性研究。使用3的信噪比（S/N）计算得出，水溶液中的检测限为9.0×10⁻⁹ M。在未经样品预处理的情况下，将该电极应用于药物制剂，与规定浓度的偏差可接受（相对标准偏差RSD = ±3.81%，n = 4）。对于更复杂的基质，开发了合适的萃取程序，尿样回收率> 90%，血清回收率> 75%。