Mahedero M C, Salinas F, Aaron J J
Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, Badajoz, Spain.
J Pharm Biomed Anal. 1994 Sep;12(9):1097-101. doi: 10.1016/0731-7085(94)e0039-4.
First-derivative and second-derivative spectrofluorimetric methods have been developed for the analysis of sulphanilamide (SAN) in milk, using an ethanol-water (60:40, v/v) medium. Linear calibration plots were obtained over a concentration range of 30-120 ppb, with correlation coefficients greater than 0.997. Relative standard deviations were within the range 1.6-3.6%. Limits of detection were 0.7-2.7 ppb. Recoveries of 90-102% were obtained. The derivative fluorescence methods are simple, rapid and sensitive and there is no interference from fluorescent impurities in the determination of low levels of SAN in milk.
已开发出一阶导数和二阶导数荧光光谱法，用于在乙醇 - 水（60:40，v/v）介质中分析牛奶中的磺胺（SAN）。在30 - 120 ppb的浓度范围内获得了线性校准曲线，相关系数大于0.997。相对标准偏差在1.6 - 3.6%范围内。检测限为0.7 - 2.7 ppb。回收率为90 - 102%。导数荧光法简单、快速且灵敏，在测定牛奶中低水平的SAN时不受荧光杂质的干扰。
