Persistence of polyomavirus in adult SCID C.B-17 mice.

C.B-17 mice with the Severe Combined Immune Deficiency (SCID) mutation were infected with the naturally occurring murine polyomavirus. Using the Polymerase Chain Reaction (PCR) technique, persistence of polyomavirus was followed in different tissues of the mice between 24 hours and 2 months post infection (p.i.). Viral DNA appeared by 3-5 days and was detected in all studied organs by 3 weeks p.i. From 4 weeks to 2 months p.i. viral DNA was present at high levels in all studied organs in all of the animals. As controls normal C.B-17 and A/Sn mice were used. Viral DNA appeared by 2-4 days. The infection reached a peak around 1 week p.i. This was followed by a clearing stage and viral DNA was no longer detectable by 4-5 weeks p.i. Most organs studied with PCR were also examined histologically, but no lesions were observed. Consequently persistence and organ distribution of polyomavirus in adult SCID mice differs greatly from that in normal adult mice.