Stout J G, Brittsan A, Kirley T L
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0575.
Biochem Mol Biol Int. 1994 Aug;33(6):1091-8.
We have investigated the relationship between the ecto-Mg-ATPase and the neural cell adhesion molecule (NCAM) in brain. Rat and chicken brain microsomes were solubilized and the crude, soluble mixtures were partially purified by anion exchange chromatography. Chromatographic fractions were assayed for the presence of Mg-ATPase activity and the ATPase and NCAM proteins by Western blot analysis. In both rat and chicken brain, the Mg-ATPase was separated from the neural cell adhesion molecule on the anion exchanger. In addition, immunoprecipitation of solubilized rat brain microsomes with an anti-NCAM antibody did not precipitate any Mg-ATPase activity but did precipitate NCAM. We conclude that the brain ecto-Mg-ATPase is neither identical to, nor closely associated with, the neural cell adhesion molecule, contrary to a previous report (Dzhangzhugazyan, K. and Bock, E. (1993) FEBS Lett. 336, 279-283).
我们研究了脑中胞外镁 - 腺苷三磷酸酶（ecto - Mg - ATPase）与神经细胞黏附分子（NCAM）之间的关系。将大鼠和鸡脑的微粒体溶解，粗制的可溶性混合物通过阴离子交换色谱法进行部分纯化。通过蛋白质免疫印迹分析对色谱馏分检测镁 - 腺苷三磷酸酶活性以及腺苷三磷酸酶和NCAM蛋白的存在情况。在大鼠和鸡脑中，镁 - 腺苷三磷酸酶在阴离子交换剂上与神经细胞黏附分子分离。此外，用抗NCAM抗体对溶解的大鼠脑微粒体进行免疫沉淀，未沉淀出任何镁 - 腺苷三磷酸酶活性，但沉淀出了NCAM。我们得出结论，与之前的一份报告（Dzhangzhugazyan, K.和Bock, E. (1993) FEBS Lett. 336, 279 - 283）相反，脑胞外镁 - 腺苷三磷酸酶既不与神经细胞黏附分子相同，也不与之紧密相关。
