Kotyk A, Georghiou G
Institute of Physiology, Prague 4, Czech Republic.
Biochem Mol Biol Int. 1994 Aug;33(6):1145-9.
Addition of univalent cations to sugar-metabolizing Saccharomyces cerevisiae, Schizosaccharomyces pombe and Lodderomyces elongisporus brought about a powerful acidification of the external medium with rates up to nearly 20 nmol H+ per min per mg dry wt. in S. cerevisiae, over 15 nmol in S. pombe, and 4.7 nmol in L. elongisporus. These rates were as much as 20 times, 5.5 times and 10.3 times, respectively. higher than in the absence of K+. Use of galactose-induced cells, of H(+)-ATPase-deficient mutants and observations over the entire growth curve indicated that the K+ effect on H+ extrusion is not connected with the H(+)-ATPase function as such but rather depends on metabolic reactions producing ATP. The effect has apparently nothing to do with the electrical potential across the plasma membrane.
向参与糖代谢的酿酒酵母、裂殖酵母和长孢洛德酵母中添加单价阳离子会导致细胞外培养基强烈酸化，酿酒酵母中的酸化速率高达近每分钟每毫克干重20纳摩尔氢离子，裂殖酵母中超过15纳摩尔，长孢洛德酵母中为4.7纳摩尔。这些速率分别比无钾时高出20倍、5.5倍和10.3倍。使用半乳糖诱导的细胞、H(+)-ATP酶缺陷型突变体以及对整个生长曲线的观察表明，钾离子对氢离子外排的影响与H(+)-ATP酶的功能本身并无关联，而是取决于产生ATP的代谢反应。这种影响显然与质膜两侧的电势无关。
