Itoh M, Kimura J, Tsukise A, Okano M
Department of Veterinary Anatomy, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Cell Biol Int. 1994 Aug;18(8):819-27. doi: 10.1006/cbir.1994.1115.
In order to detect and characterize novel molecules which function in oogenesis, immunohistochemical study using a monoclonal antibody (MAb) raised against oocytes of Xenopus laevis was carried out. The distribution of the detectable molecule with MAb X-80, which specifically reacts with Xenopus previtellogenic oocytes; stages I and II (Dumont, 1972), in particular was analyzed. The hamster, mouse, chick, quail, Caenorhabditis elegans and Lilium longiflorum were also examined. Interestingly MAb X-80 bound not only in Xenopus oocytes but in its secondary spermatocytes and spermatids. Furthermore, MAb X-80 gave a similar staining pattern in the Lilium and other, although the stage when the positive reaction is detectable is different in the male and female germ cells.
