Bishop J, Simpkins J W
Department of Pharmacodynamics, University of Florida, Gainesville 32610.
Mol Cell Neurosci. 1994 Aug;5(4):303-8. doi: 10.1006/mcne.1994.1036.
The present study provides evidence that 17-beta-estradiol (E2) exerts cytoprotective effects on both glial and neuronal cell lines. In C6 rat glioma cells, the addition of E2 to serum free media enhances live cell number by 40% at 24 h and 75% at 96 h when compared to serum free media conditions. E2 treatment of C6 cells in serum free medium did not increase thymidine uptake at any sampling time, indicating that the observed effect of E2 on C6 cell number was not due to a mitogenic effect of the steroid hormone. The addition of E2 to SK-N-SH cells in serum free media maintained both total and live cell number at a level comparable to the fetal bovine serum (FBS) treated cells at both 24 and 48 h. At 96 h after treatment with E2, total and live cell numbers were diminished relative to the 48-h sample and the 96-h FBS group, but were still more than twice the number observed in serum free media. Associated with the reduced effects of E2 at 96 h was an increase in the ratio of dead to total cells, although it remained about 50% less than the serum free group. Through 48 h, E2 exposure did not increase thymidine uptake in SK-N-SH cells, indicating that the effect of E2 on SK-N-SH cells was cytoprotective rather than mitogenic. Collectively, these data support a cytoprotective action of E2 on neuronal or glial cell types in vitro.
本研究提供了证据表明17-β-雌二醇（E2）对神经胶质细胞系和神经元细胞系均具有细胞保护作用。在C6大鼠胶质瘤细胞中，与无血清培养基条件相比，在无血清培养基中添加E2可使活细胞数量在24小时时增加40%，在96小时时增加75%。在无血清培养基中用E2处理C6细胞，在任何采样时间均未增加胸苷摄取，这表明观察到的E2对C6细胞数量的影响并非由于类固醇激素的促有丝分裂作用。在无血清培养基中向SK-N-SH细胞添加E2，在24小时和48小时时均使总细胞数和活细胞数维持在与胎牛血清（FBS）处理细胞相当的水平。在用E2处理96小时后，相对于48小时样本和96小时FBS组，总细胞数和活细胞数减少，但仍比无血清培养基中观察到的数量多两倍以上。与E2在96小时时作用减弱相关的是死细胞与总细胞的比例增加，尽管仍比无血清组低约50%。在48小时内，E2暴露未增加SK-N-SH细胞的胸苷摄取，表明E2对SK-N-SH细胞的作用是细胞保护而非促有丝分裂作用。总体而言，这些数据支持E2在体外对神经元或神经胶质细胞类型具有细胞保护作用。
