Hu S B, Nowak F V
Department of Internal Medicine, St. Louis University Medical Center, Missouri 63104.
Mol Cell Neurosci. 1994 Aug;5(4):376-81. doi: 10.1006/mcne.1994.1045.
Preoptic regulatory factor-1 (porf-1) and preoptic regulatory factor-2 (porf-2) are two novel neuropeptide genes expressed in the central nervous system and peripheral tissues. Other studies have shown that these genes may play a role in steroid-dependent brain development and functions. In this study, nuclease protection assays were employed to investigate Porf-1 and Porf-2 mRNA expression in male rat brains of different ages. The preoptic area (POA), cerebral cortex (CC), and hippocampus (HIPP) expressed both Porf-1 and Porf-2 mRNA, while only Porf-2 mRNA was detectable in the medial basal hypothalamus (MBH). Porf-1 mRNA in the POA was highest at the age of 2 months (young adult), decreased at the age of 6 months (mature adult), and remained low at the ages of 12 (middle aged) and 24 months (aged). Porf-1 mRNA in the CC was also the highest at the age of 2 months and decreased with age. However, there were no age-related changes for Porf-1 mRNA in the HIPP. Porf-2 mRNA in the HIPP was found to be low at the age of 2 months, increased at the ages of 6 and 12 months, and decreased at the age of 24 months. The effect of age on Porf-2 mRNA in the POA was similar to that seen for Porf-1, with the highest expression observed in the 2-month-old rats. There were no age-related Porf-2 mRNA changes in the MBH and CC. These results indicate differential regulation of expression of the porf-1 and porf-2 genes in the MBH, POA, HIPP, and CC. The possible roles of these two genes in maturation and aging of male rats are discussed.
视前区调节因子-1（porf-1）和视前区调节因子-2（porf-2）是在中枢神经系统和外周组织中表达的两个新的神经肽基因。其他研究表明，这些基因可能在类固醇依赖的脑发育和功能中发挥作用。在本研究中，采用核酸酶保护试验来研究不同年龄雄性大鼠脑中Porf-1和Porf-2 mRNA的表达。视前区（POA）、大脑皮层（CC）和海马体（HIPP）均表达Porf-1和Porf-2 mRNA，而在内侧基底下丘脑（MBH）中仅可检测到Porf-2 mRNA。POA中的Porf-1 mRNA在2个月龄（年轻成年）时最高，在6个月龄（成熟成年）时下降，在12个月龄（中年）和24个月龄（老年）时保持较低水平。CC中的Porf-1 mRNA在2个月龄时也最高，并随年龄增长而下降。然而，HIPP中Porf-1 mRNA没有与年龄相关的变化。发现HIPP中的Porf-2 mRNA在2个月龄时较低，在6个月龄和12个月龄时增加，在24个月龄时下降。年龄对POA中Porf-2 mRNA的影响与Porf-1相似，在2个月龄大鼠中观察到最高表达。MBH和CC中没有与年龄相关的Porf-2 mRNA变化。这些结果表明porf-1和porf-2基因在MBH、POA、HIPP和CC中的表达存在差异调节。讨论了这两个基因在雄性大鼠成熟和衰老中的可能作用。
