Takao T, Dieterich K D, Tracey D E, De Souza E B
Central Nervous System Diseases Research, Du Pont Merck Pharmaceutical Company, Wilmington, DE 19880-0400.
Brain Res. 1994 Sep 5;656(1):177-81. doi: 10.1016/0006-8993(94)91381-1.
Previous studies have demonstrated an upregulation of interleukin-1 (IL-1) receptors following treatment of mouse AtT-20 pituitary tumor cells with corticotropin-releasing factor (CRF). In the present study, we determined the modulation of IL-1 receptors and adenylate cyclase activity in AtT-20 cultures following treatment with CRF, isoproterenol, forskolin, somatostatin and dexamethasone. CRF, isoproterenol and forskolin dose-dependently increased cAMP production and [125I]IL-1 alpha binding. In contrast, somatostatin and dexamethasone significantly inhibited CRF-stimulated cAMP production and decreased both basal and CRF-mediated increases in [125I]IL-1 alpha binding. Parallel modulation of IL-1 receptors by agents that stimulate (CRF, isoproterenol and forskolin) or inhibit (somatostatin) cAMP production in AtT-20 cells suggest the importance of this second messenger in regulating IL-1 receptors.
先前的研究表明，用促肾上腺皮质激素释放因子（CRF）处理小鼠AtT-20垂体肿瘤细胞后，白细胞介素-1（IL-1）受体会上调。在本研究中，我们测定了用CRF、异丙肾上腺素、福斯高林、生长抑素和地塞米松处理后，AtT-20培养物中IL-1受体和腺苷酸环化酶活性的调节情况。CRF、异丙肾上腺素和福斯高林剂量依赖性地增加cAMP生成和[125I]IL-1α结合。相反，生长抑素和地塞米松显著抑制CRF刺激的cAMP生成，并降低基础和CRF介导的[125I]IL-1α结合增加。在AtT-20细胞中，刺激（CRF、异丙肾上腺素和福斯高林）或抑制（生长抑素）cAMP生成的试剂对IL-1受体的平行调节表明，这种第二信使在调节IL-1受体中具有重要作用。
