Sano M, Iwanaga M
Institute for Developmental Research, Aichi Colony, Japan.
Brain Res. 1994 Sep 5;656(1):210-4. doi: 10.1016/0006-8993(94)91387-0.
PC12D cells, a subline of PC12 cells, extend neurites very rapidly in response to NGF, even when RNA synthesis is blocked. Several minutes after the initiation of a concentration gradient of NGF from a micropipette in the vicinity of PC12D cells, clear projections emerged from cells on the side facing the micropipette while no significant changes in morphology were observed on the other side of cells. A control solution administered from a micropipette did not produce any changes in morphology. Longer exposure to the gradient of NGF of aggregates of PC12D cells increased the length of neurites extending toward the source of NGF. The observations indicate that the sprouting of neurites occurs locally in regions of PC12D cells that are exposed to an elevated concentration of NGF.
PC12D细胞是PC12细胞的一个亚系，即使在RNA合成被阻断的情况下，它也能对神经生长因子（NGF）做出反应，非常迅速地长出神经突。在PC12D细胞附近用微量移液器产生NGF浓度梯度几分钟后，面向微量移液器一侧的细胞出现明显的突起，而细胞另一侧未观察到形态有明显变化。从微量移液器中注入对照溶液不会引起形态上的任何变化。PC12D细胞聚集体更长时间暴露于NGF梯度下会增加向NGF来源方向延伸的神经突的长度。这些观察结果表明，神经突的萌发发生在PC12D细胞中暴露于高浓度NGF的局部区域。
