Fire A
Carnegie Institution of Washington, Baltimore, MD 21210.
Comput Appl Biosci. 1994 Jul;10(4):443-7. doi: 10.1093/bioinformatics/10.4.443.
The paper describes a digital image archiving system for time-lapse microscopy. The system uses an MS-DOS compatible computer to store video images while simultaneously controlling a stepping motor. In a typical experiment, images might be taken at 30 s intervals in each of 25 consecutive focal planes. A system with 2.5 Gbyte disk capacity can store approximately 18,000 full frame images: 6 h recording at maximum resolution. Once recorded, images series stored on disk can be 'played back' in any order. Generally, images from a single focal plane are displayed consecutively in either forward or reverse time. The focal plane can be shifted during playback, allowing individual cells to be followed as they move between focal planes. To facilitate the annotation and interpretation of the real-time images, a mouse-driven interface allows users to define and follow individual objects (e.g. cells). The recorded image series can be achieved inexpensively using standard digital tape backup hardware. In this laboratory, the system has been particularly useful for tracing embryonic cell lineages and cell migrations. Detailed system specifications, including source code, compiled programs, hardware requirements and users manual are available directly from the author or by anonymous FTP (ciw1.ciwemb.edu).
本文描述了一种用于延时显微镜的数字图像存档系统。该系统使用一台与MS-DOS兼容的计算机来存储视频图像，同时控制一台步进电机。在典型的实验中，可能会在25个连续的焦平面中，每隔30秒拍摄一次图像。一个具有2.5GB磁盘容量的系统可以存储大约18,000张全帧图像：以最大分辨率录制6小时。一旦录制完成，存储在磁盘上的图像序列可以按任何顺序“回放”。一般来说，来自单个焦平面的图像会按照时间顺序向前或向后连续显示。在回放过程中可以切换焦平面，从而能够跟踪单个细胞在不同焦平面之间的移动。为了便于对实时图像进行注释和解读，一个鼠标驱动的界面允许用户定义并跟踪单个对象（如细胞）。使用标准的数字磁带备份硬件就能低成本地获取录制的图像序列。在本实验室中，该系统对于追踪胚胎细胞谱系和细胞迁移特别有用。详细的系统规格，包括源代码、编译程序、硬件要求和用户手册，可直接从作者处获取，或通过匿名FTP(ciw1.ciwemb.edu)获取。
