Chen X Y, Ning S, Arrand J R
Department of Pathology, Guangdong Medical College, Zhanjiang.
Zhonghua Bing Li Xue Za Zhi. 1994 Aug;23(4):200-3.
Nasopharyngeal biopsies from 96 cases of clinically suspected nasopharyngeal carcinoma and chronic nasopharyngitis were investigated using EB virus DNA BamHI W fragment and EBNA-2 A, B type probes. There was a significant dominance of EBV EBNA-2 A type infection on all samples: nasopharyngeal carcinoma (NPC) 27/35 (77.1%), clinically suspected NPC 7/10 (70.0%), chronic nasopharyngitis (CNP) 18/27 (66.7%). Both W and A probes positive were 20/29 (69.0%) in NPC and 8/25 (32.0%) in non-NPC, indicating a close relationship between the infection of EBNA-2 A type virus and NPC in this series. Meanwhile, the expression of EBNA on 35 cases of biopsy were detected using the anti-complement immunofluorescence method, 19/20 (95.0%) were positive in the NPC group and 1/15 (6.7%) in the non-NPC group. Based on the positive results in detection of A, W or EBNA expression, there was a significant difference between NPC and non-NPC groups (P < 0.001). It is suggested that EBV infection, especially type A, plays an important role in the etiology and pathogenesis of NPC in the Zhanjiang area.
使用EB病毒DNA BamHI W片段和EBNA - 2 A、B型探针，对96例临床疑似鼻咽癌和慢性鼻咽炎患者的鼻咽活检组织进行了研究。在所有样本中，EBV EBNA - 2 A型感染占显著优势：鼻咽癌（NPC）组27/35（77.1%），临床疑似NPC组7/10（70.0%），慢性鼻咽炎（CNP）组18/27（66.7%）。W和A探针均阳性的在NPC组为20/29（69.0%），在非NPC组为8/25（32.0%），表明在本系列研究中EBNA - 2 A型病毒感染与NPC之间存在密切关系。同时，采用抗补体免疫荧光法检测了35例活检组织中EBNA的表达，NPC组19/20（95.0%）为阳性，非NPC组1/15（6.7%）为阳性。基于A、W检测或EBNA表达的阳性结果，NPC组和非NPC组之间存在显著差异（P < 0.001）。提示EBV感染，尤其是A型感染，在湛江地区NPC的病因学和发病机制中起重要作用。
