Litteria M
Neuroendocrine Research Laboratory, Veterans Affairs Medical Center, North Chicago, IL 60064.
Brain Res Dev Brain Res. 1994 Aug 12;81(1):113-20. doi: 10.1016/0165-3806(94)90073-6.
The effects of neonatal ovariectomy on cerebellar development and morphology were investigated in rats ovariectomized (OV) or sham-ovariectomized (SOV) 24 h after birth. At age 70 days rats were sacrificed and the cerebella processed for quantitative light microscopy of matched midline sagittal sections of the vermis. Body, brain and cerebellar weights, as well as total cerebellar area and area of the internal granular layer were significantly greater in the OV rats. The concentration (cells/mm2) and content (cells/section) of granule cells were significantly greater in each of the 10 vermal lobules of OV rats. The number of Purkinje (P) cells in the OV rats was significantly greater than control values for 6 lobules (L-III, L-V, L-VIb,c, L-VII, L-IX and L-X). P cell number for L-I, L-II and L-VIII was also higher in the OV group, but not significantly. The between group variation for P cell packing density (cells/mm) was significantly different, i.e. packing density for the OV rats was greater than the SOV value. The most consistent and significant difference between the folial patterns of SOV and OV groups was observed in L-VIII, the pyramis. A bilobed L-VIII occurred in 61.11% of SOV rats, but only in 16.67% of OV rats. Overall, neonatal ovariectomy significantly reduced the number of folia. Although the mechanisms are speculative, these data clearly indicate removal of the ovaries 24 h after birth, either directly and/or indirectly has long-term effects on cerebellar development.
研究了新生大鼠出生后24小时卵巢切除（OV）或假手术卵巢切除（SOV）对小脑发育和形态的影响。70日龄时处死大鼠，取小脑制备蚓部匹配的中线矢状切片，进行定量光学显微镜观察。OV组大鼠的体重、脑重和小脑重量，以及小脑总面积和内颗粒层面积均显著增加。OV组大鼠10个蚓小叶中颗粒细胞的浓度（细胞/mm²）和数量（细胞/切片）均显著增加。OV组大鼠6个小叶（L-III、L-V、L-VIb、c、L-VII、L-IX和L-X）的浦肯野（P）细胞数量显著高于对照组。OV组L-I、L-II和L-VIII的P细胞数量也较高，但差异不显著。两组间P细胞堆积密度（细胞/mm）的差异显著，即OV组大鼠的堆积密度大于SOV组。SOV组和OV组叶型最一致且显著的差异出现在L-VIII（锥体叶）。61.11%的SOV组大鼠出现双叶L-VIII，而OV组仅为16.67%。总体而言，新生大鼠卵巢切除显著减少了叶的数量。尽管其机制尚属推测，但这些数据清楚地表明，出生后24小时切除卵巢，无论直接还是间接，都会对小脑发育产生长期影响。
